Monthly Archives: May 2011

Protected: shadow modeling

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more flavors of y probe

more flavors of probes (DNP and Fluorescene labels).  in vitro transcription reaction setups.  

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Friday 05/13/11

10:20 A  — 4:00P, 5:00P – 8:40 P Check plates from transformations — all look excellent Final additions to thesis seminar contact Prof. Sam Kunes about fly-space Thesis Seminar Working on adding enhancer MS. Sent draft to ML.

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Thursday 05/12/11

9:40 A – 5:00 P, 8:00P – 11:35 P Gel extraction for inv E(Pc) tou and en cloning probe templates Finish yellow probe making: precipitate probes, wash, and resuspend. Finish grad div exit survey submit grad div. graduation paperwork collect … Continue reading

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cloning and probe making

inv,E(Pc),tou,en               y1-bio, y1-dig, y2-bio, y2-dig, y-full-dig-bio

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Wednesday 05/11/11

10:30A -4:00P, 8:00P-11:00P Collect virgins Flip collection cages 11:00 AM Flip collection cages 1:00 P, fix embryos: 3:00 P in vitro transcription reactions for y1-bio,y1-dig,y2-bio,y2-dig, y-full-dig-bio fusion.  started 12:00P PCR for cloning inv(e), en(e), tou(i), E(Pc)(i) for chromatin studies.  (3.20 … Continue reading

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Tuesday 05/10/11

10:10 A — 6:00 P, 8:30P – 11:00 P Working on Thesis Seminar Presentation Updating vial label printing for archives collect virgin 4X early and late New code to convert excel files to TeX readable files.  Some issues with xlsread.m … Continue reading

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Monday 05/09/11

10:15 A – 7:00 P, 8:00 P — 11:00P Getting signatures for thesis.  Susan’s signature for NSF funding + submit to Sacramento’s office. Discuss snail MS plans with Jacques, add shadow rescue data? Collect virgins AM upload Thesis Lab meeting … Continue reading

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Protected: chromatin compaction

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Sunday 05/08/11

10:30 A – 1:15 P, 8:00P-10:15P Design and order primers for en-exon, inv-exon, tou-int, E(Pc)-int (by hand, 45 min). sort and flip hb early and late rescues, clear vials for virgin collection. Flip collection cages.

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