Monthly Archives: June 2011

Progress Report 06/27/11

Posted on June 27, 2011 by admin

Progress Report Top Findings: 1. Effect of bcd does on hb expression: 6x bcd have peak hb levels similar to 2x bcd, 1x bcd are close but a little less hb and more variable between embryos. 2. hb-BAC minus shadow … Continue reading →

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Protected: mRNA counting research planning

Posted on June 27, 2011 by admin

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Thursday 06/23/11

Posted on June 23, 2011 by admin

9:20 A -4:00 P Confocal, H3K27 slides.  s04 – fluorscene stain and rab-bio antibody both work. Imaging s02, notable background in E(Pc)-dig / tou-bio. Flip all bottle stocks still in use.  Kill all UAS-possible repressor lines.  G4B lines don’t drive … Continue reading →

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Protected: MP09 intensity sensitivity

Posted on June 23, 2011 by admin

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Wednesday 06/22/11

Posted on June 22, 2011 by admin

9:40 A -3:30 p Finish ON confocal.  Data acquisition looks good, 10 embryos finished. Rerunning unsupervised dot counting with higher threshold to reduce noise in y channel on MP09_c.  (matched .05 filter rather than .02). Layout for lab-meeting.

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Tuesday 06/21/11

Posted on June 21, 2011 by admin

10:00 A — 7:00P, 7:40P – 8:20P Finish confocal run (MP09 channel flip).  Set several images first plane far too high. Working on multiple embryo data aligment for hb counting data Multi-embryo align 1x and 6x bcd hb-counts Analyzing MP09_c … Continue reading →

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Protected: bcd 1x v 6x cont

Posted on June 21, 2011 by admin

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Protected: bcd 1x and 6x counting

Posted on June 20, 2011 by admin

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Monday 06/20/11

Posted on June 20, 2011 by admin

10:00 A — 7:00P, 8:00P – 10:00P Mount slides Confocal, MP07 hets Test samples (s02, some background, weak en in test sample, need more imaging) Biophysics webpage development: added academics page and random image widget. Analyzing sna[18] MP10 data Reading … Continue reading →

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Sunday 06/19/11

Posted on June 19, 2011 by admin

3:45P – 7:20P, 8:10 P — in situs day 3 used old block on samples, then rinsed and switched to new block — don’t store diluted sample. Fixed page specific posts for biophysics page using widget logic (widget context doesn’t … Continue reading →

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