Monthly Archives: October 2011

11:15 A — make new LB and autoclave make LB -chlor, start ON culture of BAC stabs. en-BAC CH322-116J7, tou-BAC CH322-141J1, E(Pc)-BAC CH322-180C5 hand sort STORM labeled bcd embryos (7), split other tubes, post-fix all tubes 1 hr leave ON in ethanol:resin

10:45 A — 5:30 P Rinsing out primary antibody on protein stains Upgrading Linux OS to Meerkat.  Upgrading to Meerkat to Narwhale. Revising NSF proposal Bcd-A647, GFP-405:7050, hb-A555 Bcd-405:647, GFP-405:750,hb-A543 Lm-A647,GFP-405:750,hb-A555 Lm-405:647, GFP-405:750,hb-A543 rinsed out secondaries.  left at 4C over … Continue reading →

10:30 A — 9:30 P mount non-post fixed bcd-STORM 647 labeled embryos post-fix remaining embryos No apparent bcd signal viewed on epi-fluorescence from STORM 647 label. Trying direct label with Alexa-647 anti-rabbit and Alexa-555-anti sheep (to cross react with hb … Continue reading →

10:30 am — 5:50 pm, 8:00 pm – 10:00 pm Starting protein stain to test antibodies, sectioning, and STORM dyes. Santa Cruz Bicoid antibody at 1:500, hb antibody at 1:500 with blocking peptide at 1:500.  Incubating at RT 1:30P – … Continue reading →

9:30 A – 7:00 P HHMI orientation Discussion with Colenso about setting up for imaging. 750 lowest background  — good for diffuse samples.  648 with 405 activator strongest STORM dye. Colenso sent antibody staining protocol, resin imbedding protocol, (now in … Continue reading →

10:00 A — 5:00P, 8:00P – 9:00P Sebastian lab meeting Flip MP08, sna[D]/HbZ fly stocks — more emergents this time. Set up new bottle of sna[D]/HbZ for imaging Send label for embryo shipping. I9 paperwork for HHMI Mixing buffers and … Continue reading →