Daily Archives: October 25, 2011

Tuesday 10/25/11

Posted on October 25, 2011 by admin

10:00A – 8:40 P, 9:45P – 12:00A in situs day 2 yw in situ in new embryo chamber, day 1 move antibodies to opaque box in 4C under Storm2/Graham bench. use on-deck 2 mL tubes to keep hot-wash solution warm … Continue reading →

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complete fluid exchange embryo chambers 3.0

Posted on October 25, 2011 by admin

To build: cut a small gasket 3-5mm from thin plastic tubing, diameter equal to eppendorf. Fit this snuggly around a P1000 pipette tip so the resulting plastic and rubber-gasket fits very tightly in an eppendorf. Remove the inner plastic, put … Continue reading →

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Antibodies from White study

Posted on October 25, 2011 by admin

Commercially purchased: H3K9Ac ab4441 Abcam H3K9me3 ab8898 Abcam RNA Pol II 8wG16 Covance H3K4me1 ab8895 Abcam H3K4me3 ab8580 Abcam H3K27Ac ab4729 Abcam H3K27me3 07-449 Upstate H3K36me3 ab9050 Abcam HP1 ab25726 Abcam HP1 Covance a-H3K4me3-LP LP Bio a-end300 Santa Cruz

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