Daily Archives: October 12, 2011

10:40 A -6:30, 8:00P – 8:45P continue resin embedding washes.  Need light centrifuge to sediment embryos by 1:1 ethanol:resin mini-prep BACs grown O/N (16 hrs) in LB-chlor. media.  All have very low dirty yield (8-30ng/uL) probably not BAC DNA.  BACs … Continue reading →

11:15 A — make new LB and autoclave make LB -chlor, start ON culture of BAC stabs. en-BAC CH322-116J7, tou-BAC CH322-141J1, E(Pc)-BAC CH322-180C5 hand sort STORM labeled bcd embryos (7), split other tubes, post-fix all tubes 1 hr leave ON in ethanol:resin