Daily Archives: October 18, 2011

antibody labeling details

Posted on October 18, 2011 by admin

first try 647 antibody 280: .522 A405: .205 A647: .1485 second try 647 A647 =.334 + .25 offset A405=-.0768 + .25 offset antibody 280 = .4522 + .25 offset first try 750 D750 = .4889 A405 = .207 antibody = … Continue reading →

Posted in probe and plasmid building | Comments Off on antibody labeling details

complete fluid exchange embryo rinse chambers

Posted on October 18, 2011 by admin

New method for rapid and complete fluid exchange during embryo washes Cut a P1000 tip to fit snuggly inside a 1.5 mL eppendorf with the lid closed. Cut a small whole in the bottom by removing an edge off the … Continue reading →

Posted in Research Planning | Comments Off on complete fluid exchange embryo rinse chambers

Tuesday 10/18/11

Posted on October 18, 2011 by admin

8:30 A — 9:10 P Meeting with Bernhard.  Probably need to cutaway samples — try 2 micron sections with trimming knife. Review dye making with Colenso plasticize remaining samples in cylindrical mounts nicked translation round 2, try dilution series of … Continue reading →

Posted in Summaries | Comments Off on Tuesday 10/18/11