Monthly Archives: November 2011

Tuesday 11/15/11

Posted on November 15, 2011 by admin

10A – 7:40P, 9:00P – 12:15A left H3K27 K4 and polII round 2 samples at 70C since 11/8 evening . Move to 22C, see how these cut tomorrow. sign up for overnight confocal time on 710 for Wed and Thursday … Continue reading →

Posted in Summaries | Comments Off on Tuesday 11/15/11

Monday 11/14/11

Posted on November 14, 2011 by admin

10:00A – 7:00P Land in Boston 7A Lab meeting (Ke on Actin) Attempting to install drivers for graphics card GTX 590 fail badly after 3 hours of phone consultation with Dell. Can order new card from Dell with 25% discount … Continue reading →

Posted in Summaries | Comments Off on Monday 11/14/11

Thursday 11/10/11

Posted on November 10, 2011 by admin

Meeting with ML, discuss snail paper. Focus on boundary, mRNA dynamics, then heterogenity. Need figure of cc13 mRNA levels cell-by-cell graphic showing early variability.

Posted in Summaries | Comments Off on Thursday 11/10/11

Wednesday, 11/09/11

Posted on November 9, 2011 by admin

9:30 A -7:00P, 9:00P-10:00P Review / prepare presentation for Angela’s group Write paper review — responses due soonish Freeze fly-cage. Presentation to DePace group 12:30P -4:00P 2nd round H3K4, H3K27, pol II staining embryos left at 70C

Posted in Summaries | Comments Off on Wednesday, 11/09/11

basement-membrane imaging: Laminin

Posted on November 9, 2011 by admin

Confocal images in green.       Super-resolution images and super-resolution 3D reconstruction of 2 um layer below in grayscale.

Posted in Microscopy | Comments Off on basement-membrane imaging: Laminin

Tuesday 11/08/11

Posted on November 8, 2011 by admin

9:30 A – 8:30P, 10:00P – 11:40P Analyzing STORM data on Tuck working with inv/en locus data in Matlab Embryos in 70%, then pure resin for emedding.  Quite slow to drain repeated washes through the membrane filter but still works. … Continue reading →

Posted in Summaries | Comments Off on Tuesday 11/08/11

Image Analysis of en-region

Posted on November 8, 2011 by admin

  Heterochromatin in blue, E(Pc) and tou in green and red, engrailed in cyan. These are reconstructions from confocal imaging.  Still working on analysis by psf fitting and still need to do bead slide control analysis.  super-resolution imaging of chromatin … Continue reading →

Posted in Microscopy | Comments Off on Image Analysis of en-region

STORM2 3D thin-section imaging protocol

Posted on November 7, 2011 by admin

Startup Procedure Turn on laser cooling system — key switch below optics table.  For Argon/Krypton (488,647) 170C. For 750 is 1302C. Wait 15 minutes for system to gear up Turn on laser.  Wait until laser heats up (5 min) then … Continue reading →

Posted in Microscopy | Comments Off on STORM2 3D thin-section imaging protocol

Monday, 11/07/11

Posted on November 7, 2011 by admin

8:50A – 6:10P, 7:10P — 11:30P Submit fly abstracts – snail story and super-res imaging methods. embedding of H3K27 short primary embryos. Shu lab meeting Talk to Shu about CUDA implementation of optical section method described in: Mertz2011. STORM imaging … Continue reading →

Posted in Summaries | Comments Off on Monday, 11/07/11

Sunday 11/06/11

Posted on November 6, 2011 by admin

10A – 11:45A, 2:ooP-4:00P, 6:00P-11:50P Turnkey 60x works alright for mitotic sorting of DAPI staged embryos — constructed large well coverslip with channels for removing excess buffer.  In mininmal buffer embryos don’t float around and are easier to pick off … Continue reading →

Posted in Microscopy, Summaries | Comments Off on Sunday 11/06/11