Monthly Archives: November 2011

Saturday 11/05/11

Posted on November 5, 2011 by admin

2:30 P — 6:45 P chambered slides work well for checking and tracking single embryos. H3K4 works, PolII has sharp background spots but no obvious nuclear stain. Post-fix embryos and then rinse and then do sorting, prior to ethanol and … Continue reading →

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Friday, 11/04/11

Posted on November 4, 2011 by admin

10:00 A — 7:00P Draft abstract for ‘Techniques and Functional Genomics’ on ‘Super-Resolution Imaging of Regulatory Chromatin Dynamics in Developing Embryos’.  Sent to XZ.  Need to submit by Monday. Sent snail bi-stability abstract to ML.  Got feedback need to incorporated still  Rehydrating embryos to … Continue reading →

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Thursday 11/03/11

Posted on November 3, 2011 by admin

10:00 A – 7:40P, 8:10P – 11:30P Meeting with Manching, discuss new strategies for single tagged histone viewing.  (see email) Preparing bead slide for calibration of Confocal images, 1:5000 and 1:10,000 dilution in PBS w/ Mg and Ca.  Flow into … Continue reading →

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Wednesday 11/02/11

Posted on November 2, 2011 by admin

9:30 A — 7:00P, 9:00P – 11:55P Laser Safety Training 10A-11A miniprep samples: toll, y-full, neu3, sxlA, sxl3, sxl1 Restriction mapping with EcoRI — incubating at 37C at 12:30P. Expected fragmentation patterns: Neu3: 2x 2.5kb frags + 3 kb Pgem … Continue reading →

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Tuesday 11/01/11

Posted on November 1, 2011 by admin

10:30A — 10:30 P prep cover-slips.  Need more 22×33 No 1 slides for Hazen. Sectioning — 3 embryos — 1st is unfertilized or pre cycle-14, no laminin detected. Embryo #2 is cycle 14, very nice laminin.  Embryo #3 is very … Continue reading →

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