Monthly Archives: September 2013

journal club

Posted on September 9, 2013 by admin

(Sebastian presenting) Paper Lang … Chin Nature chemistry 2012. currently method of choice in the field. background site specific labeling of single cystine. Hard to remove extra Cys Fluorescent protein fusions (large, may have major functional impacts). self-labeling proteins: SNAPtag, … Continue reading →

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Protected: lab meeting 09/09/13

Posted on September 9, 2013 by admin

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Sunday 09/08/13

Posted on September 8, 2013 by admin

Goals Run gel to test RT reactions from plate-based T7 (complete) second proof read of review article, prepare to submit (not complete) STORM imaging (complete) Review started rereading and editing replies to reviewers. Results E6, E7, E10, F6 with P1 … Continue reading →

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Friday 09/06/13

Posted on September 6, 2013 by admin

8:50a – 5:10p, 8:30p – 10:40p Other stuff write to landlady about move in return keys for 2nd floor Coding fixed bug in STORMfinderBeta.m For Hao for updates: Fork fast-forward https://github.com/blog/266-fast-forward-your-fork Data Analysis copying files to ProRAID while running splitQVdax … Continue reading →

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Protected: project 2: 09/05/13

Posted on September 5, 2013 by admin

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Thursday 09/05/13

Posted on September 5, 2013 by admin

8:30a – 8:30p, 9:00p-11:50p Goals: Prep slides for meeting with XZ – complete. work on reply to reviewers – complete. Return Prob and Stats book – complete Set up splitdax analysis – running. book car for Banff – complete Themes … Continue reading →

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Wednesday 09/04/13

Posted on September 4, 2013 by admin

8:15a – 4:50p, 9:30p – 12:00a Discussions discuss project 2 troubleshooting with Hao and Jeff discuss with Jeff filtering STORM data on molecule properties (such as width photon number correlations or average intensity per flash as a function of space). … Continue reading →

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Protected: project 2: update 09/04/13

Posted on September 4, 2013 by admin

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Tuesday, 09/03/13

Posted on September 3, 2013 by admin

8:45a – 4:50p, 8:45p – 11:45p Goals re-read review — complete finish discussion — complete Stop IVT reaction, start RT reactions. Freeze RNA. — complete. talk to Hazen about fixing Hal. — fixed. call Calgary rental car. — Didn’t do. … Continue reading →

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Monday 09/02/13

Posted on September 2, 2013 by admin

8:30a – 6:00p, 8:00p – 11:10p T7 plate reaction planning (?) NEB Quick High Yield T7 template = 10 uL of T7 template (~30-120 ng/uL from spot check) 10 uL NTP buffer mix + 1uL (1/2x) T7 polII mix + … Continue reading →

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