Monthly Archives: November 2013

Protected: project 2 to do

Posted on November 8, 2013 by admin

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Thursday 10/07/13

Posted on November 7, 2013 by admin

9:25a – 11:50p Probe Making very poor probe incorporation. Maybe the lower concentration of Maxima? Try to run RT again tomorrow to compare. attempted DCC of probes to remove excess primer using short probes 1-4. Extremely low yield (20 ng/uL … Continue reading →

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Wednesday 11/06/13

Posted on November 6, 2013 by admin

8:30a – 12:20a Morning tasks give Jeff deep-seq prep’d sample 4 (F3) to run as qPCR standard All analysis tasks on Cajal and all split-dax running from Monet completed. Restart Monet to install new 4Tb HDs: ProBox5 & 6. Move … Continue reading →

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Tuesday 11/05/13

Posted on November 5, 2013 by admin

10:00a – 12:30a Probe Making New short probes D07 9 kb Green transcribed E02 13 kb green (between R/Y) E03 BLUE 13 KB (no genes, good PC/Psc) E04 BLUE 10 Kb (flanked by strong PolII) E05 BLACK/blue 54 kb region … Continue reading →

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Monday 11/04/13

Posted on November 4, 2013 by admin

8:40a – 12:50a Goals Cell culture check cells. Probably all need to grow at least another day or two Show BB the contaminated cultures for teachable moment. Cell staining ~ 3-4 pm start hot washes for Y RNA vs DNA … Continue reading →

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Protected: lab meeting 11/04/13

Posted on November 4, 2013 by admin

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Sunday 11/03/13

Posted on November 3, 2013 by admin

7:30p – 10:10p STORM Analysis E08 failed to run properly through split-dax launch STORM analysis. Running smooth at 70% on Cajal. Probably close to max read/write speed for disk drive across net. Wait to launch E08 again until tomorrow. Cell … Continue reading →

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Saturday 11/02/13

Posted on November 2, 2013 by admin

9:00a – 12:00p remotely. 12:00p – 12:00a Goals Coding Add to ChromatinCropper export labeled overview fig, object centroid and bounding box in original coordinates (for computation of distance to lamina) direct measure of distance to lamina New functionalities for ChromatinCropper … Continue reading →

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Friday 11/01/13

Posted on November 1, 2013 by admin

10:00a – 9:15p Coding ChromatinCropper Current version not saving vlists! (molecule lists specific to ROI). need more display images in analysis. Data analysis: H:\2013-09-24_F6-405\splitdax Savefolder: ‘C:\Users\Alistair\Documents\Research\Projects\Chromatin\Data\2013-11-01_coloredRegions’ SplitDax System now checks filesize of the new daxfile. If expected size = actual … Continue reading →

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