Tag Archives: STORM

Tuesday 12/11/12

Posted on December 11, 2012 by admin

10:10 A Check PCRs (in blocks 3A (sim screening) and 3B (Espl screening) better way to do this: complementation test back to original sim[D] and Espl[D] lines.  Just need to expand those lines back to bottles, will need ~100 vials … Continue reading →

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Monday 12/10/12

Posted on December 10, 2012 by admin

9:50 A -7:40P, 8:40P – 12:30 A yari lab meeting De-noising: use conventional image as low pass filter to remove non-blinking background Use conventional alignment for drift correction, translate all STORM movies to match multi-color conventional image. Use field of … Continue reading →

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Sunday 12/09/12

Posted on December 9, 2012 by admin

10:20 A – 7:30P, 9:30-11:00P STORM 750 1600 mA = 18 mW 1.45 mW 405 200 mW set 647 Made new Glox 750 not switching well.  Not sure if it’s just low labeling (405 looked okay but not strong). 647 … Continue reading →

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Monday 12/03/12

Posted on December 3, 2012 by admin

8:45 A — 7:30 P, 8:45P – 12:40 A Confocal data Finish O/N confocal run transfer files modify imviewer LSM to automatically detect all .lsm files in a folder and convert them all to TIF / labeled stacks of TIFs … Continue reading →

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Saturday, 11/24/12

Posted on November 24, 2012 by admin

11:15 A -7:30P, 9:30P – 11:45 P UltraCryo coverslip DFISH Hot washes for DFISH coverslips RT wash check sections on coverglass A.  Be very careful to keep sections covered with buffer. Staining definetely works.  Signal somewhat dimer than expected, but … Continue reading →

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STORM2 11/24/12

Posted on November 24, 2012 by admin

Laser powers 405: 1.5 mW 488: 115 mW at 45% power 561: 135 mW at 200 mW GUI/laserhead 642: 135 mW at 200 mW GUI/laserhead 750: 250mW at 1800 mA. –> too high? 750: 120 mW at 1400 mA –> … Continue reading →

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Sunday 11/18/12

Posted on November 18, 2012 by admin

10:15 A – 6:50 P, 7:50P – 10:30P Probe making have not confirmed nicking enzyme concentration for digestion.  Canceled PAGE introduction with Bryan, will attempt again later. Telomeres Read over telomere manuscript from John Wu  Imaging telomeres: 656 = 60 … Continue reading →

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Thursday 11/07/12

Posted on November 8, 2012 by admin

9:30 A -8:00P, 9:30-11:00P check STORM.  — run finished without losing focus. Summary of inv run:  Unfortunately 514 doesn’t work either as a double label. inv-Epc male a lot of promising double spots, but it’s a litter harder to sort … Continue reading →

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Wednesday 11/06/12

Posted on November 7, 2012 by admin

9:45 A – 8:30 P write to ML about bistability write to Bender about Hox model.  — apparently email contact is not recommended.  Try phone tomorrow. write summary of Francis suggestions remove medium from S2 cells, add fresh medium.  Reduce … Continue reading →

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Tuesday 11/06/12

Posted on November 6, 2012 by admin

9:15 A – 9:00P, 10:ooP-11:00P (vote, 8:45A) Washing out probe from on slide DFISH.  hot wash at 60C -forgot for 2hrs (oops!) stain with Hoechst and WGA-488 Working on presentation for Francis group meeting STORM characterizing embryo background (see images) … Continue reading →

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