Tag Archives: STORM

Saturday 08/25/12

Posted on August 25, 2012 by admin

10:30 A – 7:30P, 8:3oP – 10:40P check O/N confocal run, 13 embryos 2 channels 70 sections on speed 5 started at 8P, 12/13 finished. (several embryos were not substantially flattened, hence the extra sections). expansion of lens has changed … Continue reading →

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Friday 08/17/12

Posted on August 17, 2012 by admin

10:00 A  – 8:45P, 10:00P-11:45P Etching slide 1 from 6/25 in situs, 1 Dfd-dig, tub-bio.  25 min etch. Mostly over-etched.  setting up for STORM imaging of remaining sections:. more snail simulations. Approach 1, see if kicking in a twist activation of … Continue reading →

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Thursday 08/16/12

Posted on August 16, 2012 by admin

10:10 A-4:30P, 9:00P – 11:40 P (8A-10A volleyball practice) etching sample S7 from 6/25 (coverslip 2 from last night).  sample disolved clean away at 55 min.  (previous sample over-etched at 1hr:30, sample before that nicely etched at 1 hr 30, … Continue reading →

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Protected: tissue specific Methylation state of the BX-C complex by STORM

Posted on August 15, 2012 by admin

There is no excerpt because this is a protected post.

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Monday 08/13/12

Posted on August 13, 2012 by admin

9:00 A – 6:40P, 8:00P – 10:30P Zeiss780 training (just like 710.  use 10% of previous laser power.  Detector will shut off for protection if too much saturation) Sarah Lab meeting wnt signalsomes and purinsomes Adding mol-list to image functionality … Continue reading →

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Sunday 08/05/12

Posted on August 5, 2012 by admin

2:30P -8:30 etching S4, 1hr sample outrageously under-etched, resin edges clearly visible. Need to check these on Turnkey first.  Could even blast 647 on STORM scope. confocal of triple label try 3 successfully wrote rebuild_mosaic.m.  just use for 100x images — … Continue reading →

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Saturday 08/04/12

Posted on August 4, 2012 by admin

10:00A-2:45P, 5:20P-7:00P, 8:30P-10:30P in situs day 3 (triple stain try 2). STORM imaging slide 2 from 6/24 stains (Antp) mount samples 1-5 + centromere/K27/Dm01 v2, post-fix rest. Hal running smoothly. Imaging conventional images of each section just prior to STORM … Continue reading →

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Friday 08/03/12

Posted on August 3, 2012 by admin

9:50 A – 8:15 P STORM O/N run still scanning (ran 488 movie 2Hz too long).  Aborted Make new Glox (STORM4 images look dim / not enough photons/switches given brightness of conventional signal).  suspect Glox kept in door might have … Continue reading →

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Thursday 08/02/12

Posted on August 2, 2012 by admin

9:50A — 6:20, 8:20p – 11:55P Confocal O/N — not enough sections / .17 spacing too close to make it through nuclear layer.  trashed image. STORM O/N — Hal froze on movie 48.  Sent crash report to Hazen.  Shut down … Continue reading →

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Wednesday 08/01/2012

Posted on August 1, 2012 by admin

10:10 A – 7:30P, 8:20P – 10:45P Flip collection cages. Meeting with Ting and Xiaowei. KE miniprep cg5… cg10… and bxd samples.  lab colonies did not grow in liquid culture?  Sent samples for sequencing. STORM of Pc6_cntrl Label new dk-rb-750 … Continue reading →

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