Tag Archives: images

issues with computing photon counts by exponential distributions

Posted on July 9, 2012 by admin

Fitting exponential distributions to molecule areas output from insight, multiplied by photon per count for gain 30  on STORM2 (.41 photons).       These data are all from Hazen’s 2D microtubules.  My 3D microtubules, Yari’s 2D sections, my 2D … Continue reading →

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Friday 07/06/12

Posted on July 6, 2012 by admin

10:10 A – 7:30P, 9:00P -11:15P Flip fly cages, 10:10A Revisions of dynamics paper, revised, updated to v7. compare to NM requirements.  Version and comparison sent back to ML.  May need to combine figures and prune text. Take embryos out … Continue reading →

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Probe tests, June 2012

Posted on July 5, 2012 by admin
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last two weeks in images

Posted on July 5, 2012 by admin
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Protected: snail dose effects

Posted on May 28, 2012 by admin

There is no excerpt because this is a protected post.

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Tuesday 05/08/12

Posted on May 8, 2012 by admin

10:00A – 10:50 P Bcd Gal4 x shRNA Pc2 and x Psc shRNA1 both still hatch larvae. Flip collection cages check mail for Perrimon Gal4 3x line – not yet arrived rerunning Pc-GFP controls with Colenso’s parameters (min height 750 … Continue reading →

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Friday 05/04/12

Posted on May 4, 2012 by admin

12:00 P – 9:30 P 8:30-12P volleyball practice update probe & plasmid library update antibody labels collect virgins set up shRNA crosses x Pc shRNA2 and Psc shRNA 1 in collection cages PCR for in vitro transcription rxn to make … Continue reading →

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Wednesday 04/25/12

Posted on April 25, 2012 by admin

9:45 A – 8:30 P O/N hybridization — should do in PCR block.  Water evaporates from 37C block, not good contact with PCR tube.  Refilled water, allow another hour of hybrdization before washes. Sequencing results:  A1 = Taf1 antisense (sequence with m13F … Continue reading →

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Protected: DNA-Oligo STORM try 1

Posted on March 24, 2012 by admin

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Sunday 03/18/12

Posted on March 18, 2012 by admin

11:00 A – 8:45 P No colonies on plates Finish O/N STORM run.  Run failed at movie 121, unknown error message. attempt bead feducial drift correction: polysterene beads good autofluoresnce in all but 750, but bleach fast. 405 beads not … Continue reading →

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