Tag Archives: coding

Wednesday 09/12/12

9:20A – 7:00P STORM4 status Laser powers: 488 68mW, 561 50mW, 656 78mW. Neither table is floating At first system failed to connect properly to AOTF Fixed AOTF by reboot, but system now fails to connect to focus-lock IR camera. … Continue reading

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Coding projects to do:

mRNA counting package change fname to include slidedate folder as well as fileroot in all major scripts: Unsupervised_Dotfinding, Analyze_data, Plot_data scripts STORM analysis Matlab wrapper for multifit Daostorm (to run locally). Finished and running smoothly on Tuck, 09/16/12 Modify insightM … Continue reading

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Tuesday 09/11/12

9:10 AM – 7:15 P, 9:00P – 11:55 P Log off Tuck (Reserved for Yari) – 7 Processes all doing final fit on ~500,000 molecule list (about 1 molecule/per sec!  These are gonna have to get killed…) Finish O/N STORM … Continue reading

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Saturday 09/08/12

10:15 A – 7:00P, 9:00P-10:00P Working with new ways to plot snail data updated annotation on snail modeling and snail Pr/enh data plotting. modeling repressor strength. plotting summary of k27 STORM results at Hox loci (see post) working on plotting … Continue reading

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Thursday 09/06/12

9:50 A – 10:00 P InsightM overloads Tuck from O/N data analysis. Canceled all further submissions to que to allow memory / CPU to free up for other users during the day. InsightM also crashing itself due to memory overload … Continue reading

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Sunday 09/02/12

11:30 A – 3:00P, 5:30P-8:00P writing to mounia about sna ths getting data for mounia on sna ThsPr: cc14l, cc13 writing to Mike / Jacques / Mounia about papers m116a & b has a bit high false detection rate (higher … Continue reading

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Saturday 09/01/12

4:45 P – 7:00P Break down STORM4 O/N run on S1 AbdA AbdB Ubx Break down confocal O/N run of sog Pr. Analyzing snail Pr Ths Pr data. Fixed bug in snail_comp_promoters code: index i was being used both in … Continue reading

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Thursday 08/30/12

10:15 A – 12:00A Admin stuff: Nstar application faxed.  HHMI benefits sent to old address, can get canceled and resent after 30 days. break down STORM4 run. Organizing snail data [spreadsheet 0AjSqkxgziU1YdGxMQnMtd0ZXaDhDY09BbHY2SmZqX2c sheet=1 602 602] Fixed bug and got sample code … Continue reading

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Saturday 08/25/12

10:30 A – 7:30P, 8:3oP – 10:40P check O/N confocal run, 13 embryos 2 channels 70 sections on speed 5 started at 8P, 12/13 finished. (several embryos were not substantially flattened, hence the extra sections). expansion of lens has changed … Continue reading

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Friday 08/24/12

10:15 A – 9:30P running analysis on images sent by Mounia.   see post4160. Write up snail analysis updates (see post). Trying to fix lsm format error on image 13 and later of m106a.  Unable to ID parse error. Write … Continue reading

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