Tag Archives: STORM

Protected: H3K4me3 by confocal and STORM

Posted on February 12, 2012 by admin

There is no excerpt because this is a protected post.

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Sunday 02/12/12

Posted on February 12, 2012 by admin

9:45 A — 5:20P, 8:30P — 11:50P Finish STORM O/N of S45 (13-introns) setup STORM run of Pc Flip fly cage (10:50P) Flip fly cage (1:50P) Hal holds control of 647 laser AOTF against labview. Fixed. Analyzing STORM data by … Continue reading →

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Friday 02/09/12

Posted on February 10, 2012 by admin

10:10 A — 11:20 P move samples from 4C, change 100% EtOH STORM: setup 10x objective for finding samples. substantial offset, need to calibrate. Need to set laser powers to zero but have lasers on before starting dave! Ordered Bloomington … Continue reading →

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Monday 02/06/2012

Posted on February 6, 2012 by admin

9:45 A – 1:30A Flip fly plates, 10A lab meeting — planning STORM workshop. Fix embryos, 15 min wt fix, 2-5 hrs. 25 min MP08 fix, embryos added to todays in situs. STORM analysis to test parameters for S45 data. … Continue reading →

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Sunday 02/05/12

Posted on February 5, 2012 by admin

9:40 A – 8:30P, 10:00P – 1:00A Flip fly plates try relabeling Dm0 antibodies with cy3B and 647 (first round labels still pretty concentrated protein.  Most of this is not antibody but we just reduce the dilution when adding to … Continue reading →

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Saturday 02/04/12

Posted on February 4, 2012 by admin

11:15 A – 7:30P Flip collection cages, 11:20 A Write JTB review Aliquot new dyes label new laminin Dm0 antibodies.  Protein conc very high, low labeling frequency.  see STORM antibody record. imaging failed.  had focus lock on wrong objective for … Continue reading →

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Tuesday 01/31/12

Posted on January 31, 2012 by admin

10:00 A — 12:55 A Flip fly cages (MP08 and yw) Review JCC app. Submit JCC app. Working on snail paper final results/model section. Syncing datafiles on snail patterning sectioning: Cut 2 slides of s04(13-introns), one of slide 1, one of … Continue reading →

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3D histone views

Posted on January 20, 2012 by admin

3D reassembly of microsection stack of STORM images of individual nuclei with pan-histone marker.  Some alignment issues still, but worth a comparison

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STORM imaging progress update

Posted on January 14, 2012 by admin

Review of recent progress on multicolor STORM imaging of gene regulation. WGA labeling   membrane in 100 nm section.  little Hb detected (red stain mostly background). en-dig stains well with H3K27.  just need to cut it better. AbdA (red) and Ubx (green) … Continue reading →

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Saturday 01-14-12

Posted on January 14, 2012 by admin

11:00A – 7:50 P No engrailed stain No evidence that short fix, antibody label, then in situ, actually labels mRNA efficiently. No hb-mRNA detected. attempted imaging of en-dig (cut yesterday) 50 min etching removed all cells, only bits of yolk … Continue reading →

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