Tag Archives: confocal

Friday 07/13/12

Posted on July 13, 2012 by admin

9:50 A – 7:00P Submit kinetics paper John recommends 3 into 20 dilution of BAC probe, stain on slide in a sandwich between coverglass and a slide.  Use heat block with insert upside down for hybrdization clean up Snail Doc … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Friday 07/13/12

Thursday 07/12/12

Posted on July 12, 2012 by admin

10:15 A — 8:20 P, 9:20P – 12:00A Finish writing draft cover letter for kinetics paper.  Send to ML. in situs embedding (day 4) KE. coat new coverslips KE. update code repos (need to do this more often). working on … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Thursday 07/12/12

Wednesday 05/30/12

Posted on May 30, 2012 by admin

12:00 P -8:00P clean and coat 2 racks of small coverslips confocal analysis of embedded oligo-paint sample.  Embryos not detectable. sectioning of Su(Z)12-750 K27me3-647 + Dm0-750,647 working on new vector version STORM analysis code.

Posted in Summaries | Tagged , , | Comments Off on Wednesday 05/30/12

Saturday 04/28/12

Posted on April 28, 2012 by admin

11:00 A – 11:45 P Confocal imaging of Pc-GFP and Pc Reading up on DNA FISH from Bantignies protocol.  10 min denature probe at 95C.  15 min, denature embryos in prehybe at 80C.  Combine hot. wash 1hr at 37 with … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Saturday 04/28/12

Saturday 04/07/12

Posted on April 7, 2012 by admin

10:00 A – 7:15 P Confocal: embedded en-E(Pc)-tou-H3 sample does indeed have good intronic staining.  Not many 330 nm sections happen to contain the locus.  Plenty but still a minority of cells are clearly transcribing E(Pc).    So most sections … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Saturday 04/07/12

Wednesday 04/04/12

Posted on April 4, 2012 by admin

9:35 A – 7:30, 9:30 – 10:50 P wash out O/N antibodies Flip fly collection plates 10:10A Request more time to write review Flip expanding Gal4-Bcd3’UTR lines:  growing slowly only 2 bottles worth O/N collection plates of en-GFP, Bcd-GFP, Pc-GFP … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Wednesday 04/04/12

Tuesday 04/03/12

Posted on April 3, 2012 by admin

10:00 A – 11:00P washing out primary antibodies (after 10 hrs at 4C) maybe should do longer, return to 1 hr RT followed by O/N 4C? We’ll see how these ones look. Confocal images of embedded embryos for sectioning today … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Tuesday 04/03/12

Sunday 04/01/12

Posted on April 1, 2012 by admin

8:50 A — 11:30P Finish O/N confocal of MP08. For Zeiss710: a 1.7 um offset from center, with 8 um difference between top plane and imaging plane for the first sample only (with other samples all set to top plane) … Continue reading →

Posted in Fly Work, Summaries | Tagged , | Comments Off on Sunday 04/01/12

Saturday 03/31/12

Posted on March 31, 2012 by admin

design primers for AbdB regions Confocal imaging of MP08 slides from 02/06 in situs: focus on getting more cycle 13 embryos.

Posted in Summaries | Tagged , | Comments Off on Saturday 03/31/12

Friday 03/23/12

Posted on March 23, 2012 by admin

10:45 A – 12:30 A O/N confocal 10 um is a little too much buffer between first and last, several embryos didn’t make it all the way through.  Try again tonight. Set up new O/N confocal run 6 um difference … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Friday 03/23/12