Tag Archives: embryo labeling

Tuesday 04/03/12

Posted on April 3, 2012 by admin

10:00 A – 11:00P washing out primary antibodies (after 10 hrs at 4C) maybe should do longer, return to 1 hr RT followed by O/N 4C? We’ll see how these ones look. Confocal images of embedded embryos for sectioning today … Continue reading →

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Monday 04/02/12

Posted on April 2, 2012 by admin

9:10  A – 11:55 P Working on figure 6 for snail paper MP08 v wt Literature catch up: interesting paper from Paro group on predicting gene-expression and pausing from histone-modifying proteins alone. Download a few new articles for Lit Review … Continue reading →

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Thursday 03/22/12

Posted on March 22, 2012 by admin

9:45 A -12:20 A O/N confocal run failed horribly.  Despite warm-up, quick reset of positions, and quick calibration run, devastating z change of focus occurred during run. Rinsing out primary antibodies GPU multi-fit code still running slow (70 min+ per channel per … Continue reading →

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Wednesday 03/21/12

Posted on March 21, 2012 by admin

12:00P – 9:30P (9A-12P lab volleyball practice) in situs day 2 protein stains wash out primaries, post-fix, add secondaries. started new page, see record: Kill old bottles of MP09, yw.  Need to set up new bottles STORM imaging of 3Mb … Continue reading →

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Tuesday 03/20/12

Posted on March 20, 2012 by admin

10:00 A – 8:00P, 9:30P-10:30P In situs day 1 Flip fly cages, 10:30A Attempted sodium borohydride treatment of half of samples.  Left sealed on shaker — vials exploded, rocket off of shacker from underneath tin foil cover half way across … Continue reading →

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Monday 03/19/12

Posted on March 19, 2012 by admin

9:40A – 11:55 P Lab meeting presentation: Jeff Moffit 1 hr RT O/N primaries washing out O/N primary antibody labels ACS draft app to Matt Fly meeting receipts organized, sent to Matt Promoter synchrony paper feedback for Mounia and Jacques … Continue reading →

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Sunday 03/18/12

Posted on March 18, 2012 by admin

11:00 A – 8:45 P No colonies on plates Finish O/N STORM run.  Run failed at movie 121, unknown error message. attempt bead feducial drift correction: polysterene beads good autofluoresnce in all but 750, but bleach fast. 405 beads not … Continue reading →

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Thursday 03/15/12

Posted on March 15, 2012 by admin

9:45 A – 9:30P minor z drift issues still observed in STORM run. Time to take vertical beads for z-fit.  at 1:1000 density is a bit low. try 1:500 using ND 2-3 for z-fit bead images. Might be saturated?  Need … Continue reading →

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