Tag Archives: STORM

Thursday 10/10/13

Posted on October 10, 2013 by admin

10:00a – 8:30p, 10:00p – 12:00a Goals finish abstract for Alvaro work on slides for Stowers STORM with Bogdan finish F1, start coverslip 4 STORM data processing with Bogdan Oligo Prep G3-G5 P1A405, P2Cy3, P4Cy3 samples Hybridizations for next small … Continue reading →

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Wednesday 10/09/13

Posted on October 10, 2013 by admin

9:00a – 12:35a Goals work on Presentation for Stowers STORM Ran out of disk space, crashed O/N Run lost focus anyway immediately after position 0. Probably not enough oil. Possibly positions were chosen too far apart. Need to explain these … Continue reading →

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Monday 10/07/13

Posted on October 7, 2013 by admin

9:45a – 8:00p, 1:00a – 2:00a General Group meeting cancelled XZ meeting cancelled Ph modeling Discuss analysis methods with Jeff Moment comparisons. nmin / cv^2 way different than 1, definetely not exponentional (also obvious by visual inspection, median 4-7, mean … Continue reading →

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Thursday 10/03/13

Posted on October 3, 2013 by admin

10:00a – 7:00p, 10:00p – 11:50p Goals today prep slides for meeting with XZ 10 cycle PCR with 2 uL of product to add on NEB adapters STORM of more black regions reply to Nicole and Ajaz about Ph Literature … Continue reading →

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Wednesday 10/02/13

Posted on October 2, 2013 by admin

10:00a – 11:30p Goals today repeat plate PCR, work on technique, set up for sequencing STORM pipeline, G10 425kb black. start new small black. work on Banff Report – NO PROGRESS work on slides for XZ meeting – [Full time … Continue reading →

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Tuesday 10/01/13

Posted on October 1, 2013 by admin

9:45a-4:15p, 7:45p – 11:40p Goals Today order new cy3 and 405 primers with new secondary binding sites order new cy5 and cy7 (amine?) secondary oligos for multi-color experiments Work on Banff report sign up for qPCR training at the Bauer … Continue reading →

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Wednesday 09/25/13

Posted on September 25, 2013 by admin

9:30a – 11:15p STORM stop O/N STORM start copying data to Monet from O/N start spot-finding analysis on yesterday’s O/N splitdax F6 data. G2 data also not analyzed: set up DaoSTORM analysis on Cajal G2 bead data looks sparse — … Continue reading →

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Tuesday 09/24/13

Posted on September 24, 2013 by admin

11:00a-4:30p, 8:00p-11:00p Goals: Cell culture plate cells on Poly-Lysine coverglass (with Bogdan ?) passage remaining cells (with Bogdan ?) Prep 9/2 plate and new plate for DNA FISH. Staining O/N primary staining of Pc and anti-flag. Probe making RNA cleanup … Continue reading →

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Thursday 09/19/13

Posted on September 19, 2013 by admin

9:00a – 7:45p, 10:00p – 11:00p Writing Referee work working remotely (9a-12p) write up comments BJ review Goal: Trim down BJ review by 3 of 16 pages started trimming from beginning. 1 full page shorter. mRNA indexing human genome 22917 … Continue reading →

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Monday 09/02/13

Posted on September 2, 2013 by admin

8:30a – 6:00p, 8:00p – 11:10p T7 plate reaction planning (?) NEB Quick High Yield T7 template = 10 uL of T7 template (~30-120 ng/uL from spot check) 10 uL NTP buffer mix + 1uL (1/2x) T7 polII mix + … Continue reading →

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