Tag Archives: probe making

Tuesday 01/08/13

Posted on January 8, 2013 by admin

10:00A – 10:30P (8:30-10A lab exercise/training) Bistability First draft of deterministic model figure and corresponding text Trying to figure out correct integral to compute an energy surface from the vector field.  Not working so far. Fly work collect virgins: (now … Continue reading →

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Monday 01/07/13

Posted on January 7, 2013 by admin

9:30 A – 8:30P, Probe making Emulsion PCR of engrailed region probe at Wu lab.   Final library concentration 16 ng/uL, in 14 uL.  Should PCR amplify once. Brian will run test PCR and test gel to check library quality … Continue reading →

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Tuesday 12/04/12

Posted on December 4, 2012 by admin

9:40 A – 7:45P, 9:00P – 1:00A Dfd/tub analysis Break down O/N confocal run 3 channels, speed 6, 22 positions, 17.5 finished in 11 hour run.  contact zeiss rep Casey about changing channels between z-stacks rather than every section, should … Continue reading →

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Wednesday 11/22/12

Posted on November 22, 2012 by admin

9:10 A — 8:00P, 10:30P – 12:45 A UltaCryo collect prepped samples 10A – 2P, cutting ultra cryo sections of 1-10hr MTD DFISH-fixed embryos For next time: denser embryo packing in smaller gelatin sections Cryo protect at least 30 min … Continue reading →

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Thursday 11/23/12

Posted on November 21, 2012 by admin

10:30 A – 7:30 P Thanksgiving Fly work expand YWs Flip stocks check crosses.  double HbZ not yet emerging.  recombineering crosses looking bleak, we’ll see in anotehr 5-6 days… Probe making New library stats spin down dissolving probe.  Gently Pippett … Continue reading →

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Monday 11/19/12

Posted on November 19, 2012 by admin

9:15 A — 7:15 P, 8:15P – 1:15 A Cell labeling hot washes for probe first slide (2x at 60C) 1x at RT remove and save primary antibodies at RT for primary first slide. PBT rinses (3x soln change, 3rd … Continue reading →

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Friday 11/16/12

Posted on November 16, 2012 by admin

9:45 A – 7:00P, 8:00P – 10:30P Making summary images of SCM data. Reconcentrating / Precipitating probe prior to nicking reaction. Combined into 2 tubes. Dissolving pellets at 37 O/N.  (waiting for Nicking enzyme to continue). Nicking enzyme arrived Brian … Continue reading →

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Thursday 11/15/12

Posted on November 15, 2012 by admin

9:15 A — 6:45 P, 8:30P – 12:00A collect virgins for sim Espl[D] recombineering new cross, sna males x Sp/CyoZ; TM2/TM6 virgins clear MTDs and TM2/TM6s for virgin collection (peak emergence for latest bottles?) pulverize and refreeze Espl[D] and sim[D] … Continue reading →

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Friday 11/02/12

Posted on November 2, 2012 by admin

9:30 A – 7:48 PM, 10:50 – 11:45PM Fly work Flip collection plates on MTD x SCM, MTD, and sim[D], 9:30A Flip collection plates, sim[D] 1:15  P Collect virgin Act5-Gal4, MTDs, and sim/Espl.  Sort sim/Espl against humoral bristles. New crosses … Continue reading →

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Monday 10/29/12

Posted on October 29, 2012 by admin

10:40 A – 8:15 P lab meeting canceled due to hurricane Sandy contact ML about revisions. Got suggestions for graphical abstract, now implemented.  Waiting for response on text. contact Ting about T7 primers Plan to meet with Brian and Fred (Thursday? … Continue reading →

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