Tag Archives: confocal

Friday 12-16-11

Posted on December 16, 2011 by admin

10:00A – 6:00P, 8:00P – 9:30P successful 3D pan-histone nuclear reconstructions bcd, ftz, H3 in situs post primary reaction, day 2.  incubating in primary antibody 2nd round with in situ primaries added to solution O/N at 4C. new in situs … Continue reading →

Posted in Summaries | Tagged , , , , | Comments Off on Friday 12-16-11

Sunday 07/24/11

Posted on July 24, 2011 by admin

12:50P-1:30P Break down ON confocal of MP07Hz.

Posted in Summaries | Tagged , , | Comments Off on Sunday 07/24/11

Saturday 07/23/11

Posted on July 23, 2011 by admin

5:00P – 6:30P, 8:00P-9:00P Setup and run confocal of MP07Hz snaD embryos

Posted in Summaries | Tagged , , | Comments Off on Saturday 07/23/11

Thursday 07/21/11

Posted on July 21, 2011 by admin

8:15 A – 4:50 P Finish ON confocal sna2.8 30C hets Start confocal twiN vnd, sna.  vnd is expressed in twi null twiN sim, sna imaging.  sim is expressed in twi nulls Working on plotting rescue data with new added … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Thursday 07/21/11

Wednesday 07/20/11

Posted on July 20, 2011 by admin

9:00 A – 3:45 P, 8:00P – 9:50P, 10:50P – 11:45P Finish ON confocal of MP08Hz_snaD_b rescues ON dotfinding of MP12Hz_snaD was computed on single channel only. Need to repeat. Write to Banff participants about carpool (Mary, Gregor, Narendra, Brian).  Need … Continue reading →

Posted in Summaries | Tagged , , , , , , , | Comments Off on Wednesday 07/20/11

Tuesday 07/19/2011

Posted on July 19, 2011 by admin

8:30 A – 7:00P, 9:00P – 10:40P Finish ON confocal MP12Hz with sna/sim Start new confocal fun MP06 channel-flip Update probe database: ANB_Probes.xls (excel 95 format for Mac-Matlab excel import to work).  Match all existing probes and probe templates. Update … Continue reading →

Posted in Summaries | Tagged , , , , | Comments Off on Tuesday 07/19/2011

Monday 07/18/11

Posted on July 18, 2011 by admin

8:20A Projecting 2.8het sna sim counting data Analyzing 2.8het sna sim data Projecting MP12 Hz data Analyzing MP12 Hz data Confocal MP05Hz repeat (start warmup 2:15). Finish flipping stocks except APT eye screening 3 new bottles of AP-toll Lab meeting … Continue reading →

Posted in Fly Work, Summaries | Tagged , , , , | Comments Off on Monday 07/18/11

Sunday 07/17/11

Posted on July 17, 2011 by admin

3:ooP Finish ON confocal, vertical drift lost most of stack of MP06 Hz embryos :(.

Posted in Summaries | Tagged , | Comments Off on Sunday 07/17/11

Saturday 07/16/11

Posted on July 16, 2011 by admin

7:00 P — 10:00P Setup ON confocal, MP05Hz (from 6/20) offset 8.5 um, warmup 1.25 hrs, still drifted.  Maybe too long refinding embryo planes and running sample scans.  use more aggressive vertical offset (5 or 6).

Posted in Summaries | Tagged , | Comments Off on Saturday 07/16/11

Monday 07/11/11

Posted on July 11, 2011 by admin

8:00 A –9:30P Prep for lab meeting lab meeting presentation on hb mRNA counting data (with some sna data update) MP06b data analysis: imviewer, im_nucseg, and launch dot counting

Posted in Summaries | Tagged , , | Leave a comment