Tag Archives: images

Saturday 03/16/13

Posted on March 16, 2013 by admin

11:00 A – 7:00P Kinetic modeling STORM Analysis Switching is indeed failing on BX-C 2/20 data. Sad we still don’t have this basic data with enough depth yet. S3 cell data shows plenty of blinking out at 58 and 90K … Continue reading →

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Tuesday 03/05/13

Posted on March 5, 2013 by admin

9:50 A – 7:30 P, 8:30 P – 10:50 P General Discussing replies for Banff invites. Student substitutions add to bottom of list Still need to write more acknowledgement of replies. STORM Analysis launch analysis of all unprocessed datafiles. ESATA … Continue reading →

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embryo sections

Posted on March 5, 2013 by admin

some sections lie nice and flat, others get substantial ripples. Is this knife compression? too large a section in x-y? (seems to correlate). Too thick a section? (seems also to be true in my experience).

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Monday 03/04/13

Posted on March 4, 2013 by admin

9:45 A – 7:30 P, 9:00 P-11:30 P General stuff Group meeting: see notes send out invites to Banff workshop Continue working on writing referee review Fly work flipped all bottle stocks tossed all Mat alpha tub bottles currently expanding: … Continue reading →

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Tuesday 02/19/13

Posted on February 19, 2013 by admin

10:00A – 1:40P, 3:30P-12:10A Computer restart required for hotswap and to switch Alistair8 back to eSATA delete HB data from Alistair3b (now on Alistair3). disconnect Alistair3 future disk shuffling of earlier data might be better done on Tuck? remove Buffalo … Continue reading →

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improved cluster analysis

Posted on February 18, 2013 by admin

search radius cluster algorithm fastest implimentation is hist3 with binsize = search radius can easily require multiple molecules in the search radius by filtering: M=hist3([x,y],[Xmin:radius:Xmax,Ymin:radius:Ymax]); so if we want 15 nm search radius, just make 15nm steps M>3 require at … Continue reading →

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Protected: cluster analysis first try

Posted on February 18, 2013 by admin

There is no excerpt because this is a protected post.

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Friday 02/15/13

Posted on February 15, 2013 by admin

9:30 A STORM Finish O/N run crashed hal when computer memory filled up. Need more external harddisks! Should do a test run on if we can save data on the fly from Hal over the network straight to Monet. Still … Continue reading →

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Thursday 02/14/13

Posted on February 14, 2013 by admin

9:20 A -7:00P, 9:00P – 10:30P STORM Finish O/N confocal More cells not in TIRF – sections not bleaching, even 647. Dropped TIRF angle to 14.2, now switching well, still good contrast. Might need to re-image this slide later today. … Continue reading →

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3D chromatic warp: Insight vs DaoSTORM

Posted on February 13, 2013 by admin

Results using 2013-13-02 Bead Data: Z-calibrations for this data set look pretty similar Comparing Chromatic warping DaoSTORM is actually getting a better fit and tighter warp, but not managing to get the same dynamic range in Z — only +/- … Continue reading →

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