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Tag Archives: data analysis
Friday 08/31/12
9:30 A – 7:30P, 10:30 – 12:50A Break down O/N mp10a run. save mp07 data. All look good, 7 images finished completely, last image starts at the top of the mRNA field. etching S1 AbdA, AbdB, Ubx, K27me3. Issues booting … Continue reading
Thursday 08/30/12
10:15 A – 12:00A Admin stuff: Nstar application faxed. HHMI benefits sent to old address, can get canceled and resent after 30 days. break down STORM4 run. Organizing snail data [spreadsheet 0AjSqkxgziU1YdGxMQnMtd0ZXaDhDY09BbHY2SmZqX2c sheet=1 602 602] Fixed bug and got sample code … Continue reading
Protected: sim sna thresholds
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Posted in Shadow Enhancers, snail patterning
Tagged data analysis, MP12, mRNA counting, sna, sna2.8
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Thursday 07/14/11
8:10 A – 4:45P Start flipping fly stocks (1-50 flipped) Mean wt sna levels /slide vary between 150 and 250. Project MP10_b data. 1 duplicate and 1 part bleached image. 8 good embryos. brk on Y and brk deletion lines … Continue reading
Protected: MP06b (cc13 no shadow)
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Posted in Shadow Enhancers
Tagged data analysis, MP06, mRNA counting, sna, y
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Tuesday 07/12/11
8:15 A — tried high threshold on high consecutive layers for MP06 counts, need to repeat with standard trial set of inputs. Rewriting hb-curve plotting to compute means of multiple gradients (using interp1 so curves can be matched on x-axis). … Continue reading
Thursday 07/07/2011
8:30 A — 4:50 P working on Plot_hb_data: match embryos based on endogenous gradients Reprocessing MP09 data, the .05 for speed on wt is a little too high, causes some dot loss, drop to a more conservative .03 or .01. … Continue reading
Wednesday 07/06/11
9:30 A – 4:00P Finish ON confocal of MP06Hz, 10 embryos completed (scan started at 8P) Update data on MP10_sna18, run v3 through analysis. Changing threshold indeed does not substantially alter counts. keep v4. Working on sna plotting. Plotting distributions … Continue reading
Posted in Summaries
Tagged confocal, data analysis, figures, mRNA counting, presentations, sna
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Sunday 06/03/11
3:00P – 7:00 P Transfer and analyze sna2.8Hz data. Working on lab meeting presentation.
Tuesday 06/28/11
9:30 A-7:00P, 7:40-8:40, 10:00P- 11:10P Data alignment Flip sna[D] and hb[D] flies into new bottles, keep at 25C. Compare hb data by image type. confirm fairly dynamic expression prevents easy super-position of patterns during cc13. February’s data format doesn’t fit … Continue reading