Tag Archives: embryo collection

Wednesday 10/17/12

Posted on October 17, 2012 by admin

9:10 A – 6:00P, 9:30P – 11:20 P start thawing DNA Fix Mix at 37C email BIRS team about NIH funding option update Need to coat new coverslips.  Contacting olympus about #1.5 vs #1 coverslips.  (Zeiss recommends only 1.5, strong … Continue reading →

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Thursday 08/02/12

Posted on August 2, 2012 by admin

9:50A — 6:20, 8:20p – 11:55P Confocal O/N — not enough sections / .17 spacing too close to make it through nuclear layer.  trashed image. STORM O/N — Hal froze on movie 48.  Sent crash report to Hazen.  Shut down … Continue reading →

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Wednesday 08/01/2012

Posted on August 1, 2012 by admin

10:10 A – 7:30P, 8:20P – 10:45P Flip collection cages. Meeting with Ting and Xiaowei. KE miniprep cg5… cg10… and bxd samples.  lab colonies did not grow in liquid culture?  Sent samples for sequencing. STORM of Pc6_cntrl Label new dk-rb-750 … Continue reading →

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Tuesday 07/31/12

Posted on July 31, 2012 by admin

11:00 A – 7:40P, 8:20P-11:00P (volleyball practice 9A-11A) Flip fly cages KE fix embryos 2-6 hrs old (Pc-GFP and YW) Reorganizing text for kinetics paper: homogeneity, dynamics and kinetics, dosage compensation. Adapt figure_sna_dynamics to show spatial profiles over time. STORM centromere … Continue reading →

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Friday 07/06/12

Posted on July 6, 2012 by admin

10:10 A – 7:30P, 9:00P -11:15P Flip fly cages, 10:10A Revisions of dynamics paper, revised, updated to v7. compare to NM requirements.  Version and comparison sent back to ML.  May need to combine figures and prune text. Take embryos out … Continue reading →

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Tuesday 06/26/12

Posted on June 26, 2012 by admin

10:30 A -5:00P, 9:00P – 11:30P in situs day 2 short fix 2-14 hour yw and Pc embryos. rehydrate and stain 2-14 hour short fix embryos with Su(Z)12.  Pc-GFP embryos also stained with ch a-GFP. vball lost. Synthesized Bac 1 … Continue reading →

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Friday 06/08/12

Posted on June 8, 2012 by admin

10:00 -8:00P, 9:00P -11:20P Flip fly cages Finish clean up O/N STORM run.  Coopying files from Alistair5 to Alistair4. move in situ probe-test slides into boxes.  (Move archive boxes into 4C bin to make more space in 4C drawer?) 11:00 … Continue reading →

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Wednesday 04/04/12

Posted on April 4, 2012 by admin

9:35 A – 7:30, 9:30 – 10:50 P wash out O/N antibodies Flip fly collection plates 10:10A Request more time to write review Flip expanding Gal4-Bcd3’UTR lines:  growing slowly only 2 bottles worth O/N collection plates of en-GFP, Bcd-GFP, Pc-GFP … Continue reading →

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Monday 04/02/12

Posted on April 2, 2012 by admin

9:10  A – 11:55 P Working on figure 6 for snail paper MP08 v wt Literature catch up: interesting paper from Paro group on predicting gene-expression and pausing from histone-modifying proteins alone. Download a few new articles for Lit Review … Continue reading →

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Monday 03/19/12

Posted on March 19, 2012 by admin

9:40A – 11:55 P Lab meeting presentation: Jeff Moffit 1 hr RT O/N primaries washing out O/N primary antibody labels ACS draft app to Matt Fly meeting receipts organized, sent to Matt Promoter synchrony paper feedback for Mounia and Jacques … Continue reading →

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