Tag Archives: images

Monday 05/20/13

Posted on May 20, 2013 by admin

9:20 A – 7:00P, 9:30P — 12:20 A Lab meeting visiting post-doc candidate: notes Probe making figure out increased library concentrations for large scale PCR for B/Y/K/G libraries set up 5 mL scale PCR for B/Y/K/G using nick-containing primers Setup … Continue reading →

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Sunday 05/19/13

Posted on May 19, 2013 by admin

10:30 A – 10:00 P, 11:00 P – 12:15 A Probe extraction Previous test gel, suggesting some samples at least have probe: Run denaturing gel at 100 uL scale 95 minutes. Cut out smallest band over 50 bp. sample loading … Continue reading →

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Tuesday 05/4/13

Posted on May 14, 2013 by admin

10:00 A – 5:00 P, 9:00 P — 11:45 P Analysis of signal and background in different hybe conditions Discussion with Brian on temperature results Additional notes not yet sent: Hybe conditions that reduce binding to reduce background seem to … Continue reading →

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Protected: more probe making approaches

Posted on May 14, 2013 by admin

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Protected: Ph FLAG and Ph FLAG M

Posted on May 7, 2013 by admin

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Monday 06/05/13

Posted on May 6, 2013 by admin

9:30 A – 7:45 P, 8:45P – 11:55 P Oligopaints Running out gradient gels (one at a time) hotter is better with mis-priming. using 20 bp PPT primer and library specific NP primer: with adapters: Check Ph stains booked confocal … Continue reading →

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denaturing diagnostic gels

Posted on April 14, 2013 by admin

Gel 1, 5% Agarose, 200 V in sodium-Borax (300 V melts gel). Gel 2, 15% Urea, 120V 2 hrs Gel 3 15% Urea gel, 120 V 50 min in 37C room Gel 4, 10% precast Urea denaturing gel, 120 V, … Continue reading →

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Protected: issues with ph-FLAG round 1

Posted on April 5, 2013 by admin

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Tuesday 03/26/13

Posted on March 26, 2013 by admin

10:30 A – 7:30 P, 8:30 P – 10:30 P Tx-review working on literature references, laying out introduction. Stinchcombe model write out probability mass function of discrete mRNA arrivals in terms of the random differential equation for the continuous mRNA … Continue reading →

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Protected: preliminary BX-C embryo

Posted on March 25, 2013 by admin

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