Tag Archives: in situs

Tuesday 06/12/12

Posted on June 13, 2012 by admin

10:00 A-5:00P, 9:30P – 12:00A Flip collection cages, 10:30A still working on z-calibration bug.  updated write_minimal_dax_ini file seems to be the only script that won’t run correctly.  Putting max_displacement = 1 causes errors in otherwise functional script.  May need to … Continue reading →

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Monday 06/11/12

Posted on June 11, 2012 by admin

10:00 A – 7:00P, 8:30P-11:30P Hao lab meeting in situs day 1 (by KE): protein structure between hox targets Fixing AOTF problem with STORM2.  Between beam shape and AOTF, no usable light is passed. AOTF removed from table. analyze probe … Continue reading →

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Thursday 06/07/12

Posted on June 7, 2012 by admin

10:20 A – 6:50P, 8:30P – 11:20P in situs day 3 with KE. 17 slides mounted to test tomorrow [slideshow 0AjSqkxgziU1YdG90a29UaU5ua1FLUXZ5LTFmM1pMSlE 600 300 sheet=7] Prep STORM slide of Su(Z)12-750, K27-647 STORM imaging of Pc-GFP slide Beads arrays in 3 focal planes … Continue reading →

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Tuesday 06/05/12

Posted on June 5, 2012 by admin

10:00A – 8:00P in situs day 1 with KE: new large cage of Pc-GFP and Yw Flip 3x Mat-Gal4 into new bottles.  Moved to 25C. troubleshooting chromatic warp issues in new vector storm code.

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Tuesday 03/20/12

Posted on March 20, 2012 by admin

10:00 A – 8:00P, 9:30P-10:30P In situs day 1 Flip fly cages, 10:30A Attempted sodium borohydride treatment of half of samples.  Left sealed on shaker — vials exploded, rocket off of shacker from underneath tin foil cover half way across … Continue reading →

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Saturday 02-25-12

Posted on February 25, 2012 by admin

10:45 A — 6:00P, 8:00P — 12:45 A in situs day 3 working on 3D data analysis Calibrated 750 channel from bead images Confocal: image S2 330 nm (looks like intronic en stain and mature mRNA detectable). Confocal: image Psc-hox … Continue reading →

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Friday 02/24/12

Posted on February 24, 2012 by admin

11:00A – 2:20A humidifying sectioning area.  Trimming 4H sample (all for H3 antibodies). 2 hr hot wash of embryos kept strict, followed by PBT rinse, long block (2 hrs+), then primaries 1 hr at RT, then O/N at 4C. taking … Continue reading →

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Thursday 02/23/12

Posted on February 23, 2012 by admin

10:05 A – 12:10A PcG and Bcd staining embryos incubating in primary at RT for final hour. Flip fly plates Discussion with John about projects Need to look up Drosophila repeat sequences for probe testing. in situs day 1 on … Continue reading →

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Saturday 02/19/2012

Posted on February 20, 2012 by admin

10:00A – 5:00P washing out final antibody from in situs. embryos moved to ethanol WGA staining Hox in situs 1:10 set up to try cutting thicker samples.  Using Yari’s new walled area for microtome. select Images for Cell feature on … Continue reading →

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Friday 02/17/12

Posted on February 19, 2012 by admin

10:10 A — 6P, 8P-12:30P Finish O/N STORM run Analyze STORM data collect virgins working on alignment for STORM data.  Rigid alignment seems better for the moment. staining embryos, in situs day 3. New scheme for PcG and histone doubles: … Continue reading →

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