Tag Archives: in situs

Thursday 02/16/2012

Posted on February 16, 2012 by admin

9:40 A — 12:10 A collect virgins O/N STORM run did not finish on schedule (68 of 112 images done) contact Wu lab about choice DNA FISH regions install GTX580 card. install latest NVIDIA drivers received Rad21 antibodies in 50% … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Thursday 02/16/2012

Wednesday 02/15/12

Posted on February 15, 2012 by admin

9:45 A — 11:00P Etching Dm0-1 slides (25-30 min) for STORM.  (Slide 69 in records) Flip fly cage, 10A Washing out primary antibody in PcG stained embryos in situs for PcG stained embryos: hox and en locus (see records) organized … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Wednesday 02/15/12

Saturday 01/21/12

Posted on January 21, 2012 by admin

8:45 A — 2:00 P in situs moved through resin, dense collection of embryos 10 embedding capsules of embryos made for each of the 4 in situ tubes (+4 of tube 1 by KE for practice). in situ embryos baking … Continue reading →

Posted in Microscopy | Tagged , , | Comments Off on Saturday 01/21/12

Tuesday 01-17-12

Posted on January 17, 2012 by admin

9:30A – 7:00 P in situs day 1 + training KE Flip Fly cages 10:45 A Flip collection cages: 12:45 P 3:45PM fix embryos (2-5hr MP08 collection) working on figure for fellowship app

Posted in Summaries | Tagged , , , , | Comments Off on Tuesday 01-17-12

Saturday 12-17-11

Posted on December 17, 2011 by admin

12:00P – 1:00 P, 8:45P – 10:20 P O/N confocal only finished 12 embryos between 8p and noon, extended run to finish remaining 4 selected on slide. mount all MP08.  mount subsets of embryos: eng-dig, eng-fl, antp+ubx, AbdA+ubx. remaining embryos … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Saturday 12-17-11

Friday 12-16-11

Posted on December 16, 2011 by admin

10:00A – 6:00P, 8:00P – 9:30P successful 3D pan-histone nuclear reconstructions bcd, ftz, H3 in situs post primary reaction, day 2.  incubating in primary antibody 2nd round with in situ primaries added to solution O/N at 4C. new in situs … Continue reading →

Posted in Summaries | Tagged , , , , | Comments Off on Friday 12-16-11

Thursday 12-15-11

Posted on December 15, 2011 by admin

7:45 A – 8:30 P stopped O/N confocal run, only finished 6 embryos (11P recording start until 7:45A stop). hot washes for in situs (engrailed, ubx, antp, and MP08) split bcd, ftz, H3K4 embryos, start in situs with half of … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Thursday 12-15-11

Sunday 06/19/11

Posted on June 19, 2011 by admin

3:45P – 7:20P, 8:10 P — in situs day 3 used old block on samples, then rinsed and switched to new block — don’t store diluted sample. Fixed page specific posts for biophysics page using widget logic (widget context doesn’t … Continue reading →

Posted in Summaries | Tagged , , , | Leave a comment

Saturday 06/18/11

Posted on June 18, 2011 by admin

2:30P – 7:00 P, 8:00P – 10:30 P in situs day 2 Analyze s07_MP08Hz data Finish confocal MP09Hz_c write response for Stas to stats paper

Posted in Summaries | Tagged , , | Comments Off on Saturday 06/18/11

Friday 06/17/11

Posted on June 17, 2011 by admin

10:00 A — 7:40P, 8:10P — 10:00P Send response letters to PIs Plan out next round of in situs in situs day 1 renamed Enhancer_modeling project to mRNA_counting as more appropriate description.  Fixed most-all links in m-code.  Still need to … Continue reading →

Posted in Summaries | Tagged , | Leave a comment