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Tag Archives: Ph
Protected: Ph project update
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Tuesday 01/07/14
10:00a – 10:15p chromatin data collection Finish O/N STORM on F04. Transfer data from F03 and F04 to Monet start analysis of F03 and F04 data wash out stains for new BX-C regions: F05, G04 (ANTC), F03-F04-F05 combo, F05-A647 F06-A750. … Continue reading
Thursday 01/02/2014
9:30a – 9:00p Ph project Finish O/N STORM run Take IR bead images (slide is also getting clumpy. Maybe we need to stick these down with EDAC. I think making slides for long term storage with Glox is also a … Continue reading
Wednesday, 01/01/2014
1:30p – 9:10p, remotely, 12:30a – 1:15a Goals more Ph imaging Wt New Year’s letter website updates Troubleshooting drift correction curves. start working on dual-color ChromatinCropper STORM for Ph project The buffer has all gone, because I ran the thing … Continue reading
Tuesday 12/31/13
9:00a – 5:00p Goals set up STORM imaging for PhWtPh. improved drift correction write quick script to average bead dax movies (and split them out?). Test on the G1-G1 0004 data? modify feducialDriftCorrection function to be able to handle time … Continue reading
Saturday 12/28/13
9:30a – 1:45a Library 3 Design / probe building Results from first attempt at probe assembly oligoArrayCommand was getting rewritten in the loop, need a temp parameter to preserve the genename flag to be used for substitution consequently probe building … Continue reading
Thursday 12/12/13
10:45a – 11:00p Ph Simulations Troubleshooting extra along bonds. Oops, actually just resulted from plotting the wrong attachment matrix for the given position list. Simulations ran at subsaturating Ph — more total sites than available Ph. Hence there is a … Continue reading
Posted in Summaries
Tagged cell labeling, coding, Ph, troubleshooting
Comments Off on Thursday 12/12/13
Wednesday 11/27/13
Goals Sequencing analysis plots of coverage uniformity per probe set plots of failed probe vs. purity using new indexed method (or maybe just a length cut-off) Ph Project restart simulations Genomics start building library 4 (on Monet) start building library … Continue reading
Tuesday 11/19/13
9:15a – 1:00a Goals Slides for meeting with Levine Chromatin STORM analysis Analyze D11 region cells should arrive today — start in culture Library 3 design Library 3 observation: short green regions (<50 kb, basically everything not near a centromere/telomere) … Continue reading
Wednesday 11/13/2013
10:00a – 7:15p Other Reply to BJ with final corrections / proofs Deep sequencing analysis scatterplot correlations on log10 scale Coding Interesting matlab fact: Nested functions have access to the parent workspace. Got CIGAR and MD parser from Jeff to … Continue reading