Tag Archives: STORM

Monday 12/30/13

Posted on December 30, 2013 by admin

9:20a -7:00p Goals Analyze 2-color STORM data test out new image-writers for hal Getting started Monet windows explorer froze and got killed. Requires restart. Finish up notes from yesterday, add gel images to notebook. STORM attempted software update to do … Continue reading →

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Sunday 12/29/13

Posted on December 29, 2013 by admin

9:00a – 12:15a Goals Write to Ajaz about new plans Read in _oligo.txt files and analyze probe distributions STORM G1-G2 Start analysis of F12-G01 and F12-G11 data continue work on STORMrender Manual Literature Reading Kieffer-Kwon et al Cell 2013. link … Continue reading →

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Saturday 12/28/13

Posted on December 28, 2013 by admin

9:30a – 1:45a Library 3 Design / probe building Results from first attempt at probe assembly oligoArrayCommand was getting rewritten in the loop, need a temp parameter to preserve the genename flag to be used for substitution consequently probe building … Continue reading →

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Friday 12/20/13

Posted on December 19, 2013 by admin

10:00a – 12:45a STORM imaging need images of 715 beads to align A750 and A647 channels Making new 715 bead slide at 1:20,000. If this looks good we can try a 2-color bead slide as well. trimming the tape so … Continue reading →

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Monday 12/16/13

Posted on December 16, 2013 by admin

9:50a – 1:00a Group meeting 10a – 2p see notes Probe making cy7 dye from Lumipore arrived A750 labeled IDT primers arrived first test gel — try old gel of Bogdan’s (11/18). Ran smeary and fast, primers ran off the … Continue reading →

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Thursday 12/05/13

Posted on December 5, 2013 by admin

9:30a – 12:20a Goals Restart analysis for project 2 Probe making: check PCRs, continue if good, restart if not. launch STORM analysis for last few days of imaging project 2 see notes. Chromatin Project Probe Making Test PCRs Clean, reorganize, … Continue reading →

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Wednesday 12/04/13

Posted on December 4, 2013 by admin

9:40a – 11:30p Goals show Hao new project 2 results RNase-Away / bleach treat everything on my bench fix and stain new RNA cells. Allow 1 hour prehybe. Project 2 See notes Chromatin colors, double stain prep Probe organization reorganized … Continue reading →

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Tuesday 12/03/13

Posted on December 3, 2013 by admin

9:15a – 11:15p Goals Call Dad, happy birthday Analyze Ph polymer simulations for meeting with Ting. STORM Finish D07 on STORM2 (9 kb green, transcribed, RNaseA treated). Looks transcribed – variable spots, larger than expected for only 9 kb maybe? … Continue reading →

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Monday 12/02/13

Posted on December 2, 2013 by admin

9:00a – 12:20a Goals work on data analysis / slides for meeting with Ting tomorrow new round of yellow chromatin RNA DNA double stains. Include attempt restain E10rna. Still needs RNase. 3D z-calibration [POSTPONED] Start statistical analysis of Blue Polymer … Continue reading →

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Sunday 12/01/13

Posted on December 1, 2013 by admin

9:30a – 7:45p, 10:00p – 12:15a Genomics Cajal slowing down (2536 of 4385. Though all 4 variants of stringency is 4x 4385) CPU cap semi-respected, code is waiting for free cpu dips to launch new jobs. Target is 75%. Running … Continue reading →

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