Category Archives: Microscopy

whole-mount tests 02/09-10/12

Posted on February 10, 2012 by admin

slide H1: 4 color anti-nuclear lamin test   slide H2: Directly labeled antibodies: Dm01-488(green) H3K27me3-Cy3B (red), H3-Cy5 (blue), H3K27ac-750 (not shown) slide H4: H3K27me3-Cy3B (red) and H3K4me3-Cy5 (blue) along with 405 background (green). slide H4: earlier embryo: H3K27me3 ‘clusters?’  (red) … Continue reading →

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Saturday 01/21/12

Posted on January 21, 2012 by admin

8:45 A — 2:00 P in situs moved through resin, dense collection of embryos 10 embedding capsules of embryos made for each of the 4 in situ tubes (+4 of tube 1 by KE for practice). in situ embryos baking … Continue reading →

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3D histone views

Posted on January 20, 2012 by admin

3D reassembly of microsection stack of STORM images of individual nuclei with pan-histone marker.  Some alignment issues still, but worth a comparison

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hunchback and bcd antibody testing

Posted on January 12, 2012 by admin

some quick checks looking at dynamically refinement of hb gradient. Hb (green) and Bcd (red) double stained (early cc13?)         zoom , showing subnuclear localization patterns.           showing very gradual hb transition   … Continue reading →

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Protected: H3K4 imaging

Posted on December 1, 2011 by admin

There is no excerpt because this is a protected post.

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Protected: Analyzing new cc13 data

Posted on November 30, 2011 by admin

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GPU mttubule imaging

Posted on November 23, 2011 by admin
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basement-membrane imaging: Laminin

Posted on November 9, 2011 by admin

Confocal images in green.       Super-resolution images and super-resolution 3D reconstruction of 2 um layer below in grayscale.

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Image Analysis of en-region

Posted on November 8, 2011 by admin

  Heterochromatin in blue, E(Pc) and tou in green and red, engrailed in cyan. These are reconstructions from confocal imaging.  Still working on analysis by psf fitting and still need to do bead slide control analysis.  super-resolution imaging of chromatin … Continue reading →

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STORM2 3D thin-section imaging protocol

Posted on November 7, 2011 by admin

Startup Procedure Turn on laser cooling system — key switch below optics table.  For Argon/Krypton (488,647) 170C. For 750 is 1302C. Wait 15 minutes for system to gear up Turn on laser.  Wait until laser heats up (5 min) then … Continue reading →

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