Category Archives: probe and plasmid building

Saturday 11/10/12

Posted on November 10, 2012 by admin

10:45 A – 8:00P Snail project stuff Collect virgin Pr/Tm3Z, Sp/CyOZ, and TM2/TM6 (males + virgins) Order primers for screening E(spl) and sim[D].  Also for en and inv introns DNA FISH probe design Wrote new matlab script for concatinating text … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , | Comments Off on Saturday 11/10/12

Friday 11/02/12

Posted on November 2, 2012 by admin

9:30 A – 7:48 PM, 10:50 – 11:45PM Fly work Flip collection plates on MTD x SCM, MTD, and sim[D], 9:30A Flip collection plates, sim[D] 1:15  P Collect virgin Act5-Gal4, MTDs, and sim/Espl.  Sort sim/Espl against humoral bristles. New crosses … Continue reading →

Posted in Fly Work, probe and plasmid building, Summaries | Tagged , , | Comments Off on Friday 11/02/12

Thursday 10/25/12

Posted on October 25, 2012 by admin

9:50 A – 7:00P, 8:30P – 11:20 P Clean and coat coverslips washout fix from O/N fix samples, move to EtOH rehydrate, check for presence of staining. Respond to team about NSF BIRS proposal Pol I arrived 4:00 PM check … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , , | Comments Off on Thursday 10/25/12

Monday 10/22/12

Posted on October 22, 2012 by admin

9:20 A – 8:30, 9:30 – 11:10PM Flip collection cage take embryos out of 4C, final hour incubating at RT before washes. snail in situs Draq5 stained and mounted STORM / section in situs / DFISH all in secondaries at … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , | Comments Off on Monday 10/22/12

Wednesday 08/01/2012

Posted on August 1, 2012 by admin

10:10 A – 7:30P, 8:20P – 10:45P Flip collection cages. Meeting with Ting and Xiaowei. KE miniprep cg5… cg10… and bxd samples.  lab colonies did not grow in liquid culture?  Sent samples for sequencing. STORM of Pc6_cntrl Label new dk-rb-750 … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , , , | Comments Off on Wednesday 08/01/2012

Probe tests, June 2012

Posted on July 5, 2012 by admin
Posted in probe and plasmid building | Tagged | Comments Off on Probe tests, June 2012

Wednesday 05/09/12

Posted on May 9, 2012 by admin

12:30 P – 9:45 (volleyball practice 8:30A-11:30A) Pellet and resuspend all new probes. updated probe & plasmid database with new & resynthesized probes: [spreadsheet 0AjSqkxgziU1YdHlnZmFzOVBsX0JQUjlrQnRUWjVYVGc 600 400 sheet=1] 3x maternal Gal4 flies arrived from Perrimon lab -> start expanding coding molecule … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , | Comments Off on Wednesday 05/09/12

Tuesday 05/08/12

Posted on May 8, 2012 by admin

10:00A – 10:50 P Bcd Gal4 x shRNA Pc2 and x Psc shRNA1 both still hatch larvae. Flip collection cages check mail for Perrimon Gal4 3x line – not yet arrived rerunning Pc-GFP controls with Colenso’s parameters (min height 750 … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , , | Comments Off on Tuesday 05/08/12

Thursday 05/26/12

Posted on April 26, 2012 by admin

10:00 A – 11:45 P Check colonies from Bx-C round 1 cloning: all plates look good, no lawns. Gel-extract linearized Taf, Tub Gel-extract newly cloned Bx-C probes Antibody synthesis: antibodies 61-69 Flip collection cages: 10:15 A Washing out primary antibody … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , , | Comments Off on Thursday 05/26/12

Wednesday 04/25/12

Posted on April 25, 2012 by admin

9:45 A – 8:30 P O/N hybridization — should do in PCR block.  Water evaporates from 37C block, not good contact with PCR tube.  Refilled water, allow another hour of hybrdization before washes. Sequencing results:  A1 = Taf1 antisense (sequence with m13F … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , | Comments Off on Wednesday 04/25/12