Monthly Archives: November 2011

Wednesday 11-23-11

Posted on November 26, 2011 by admin

10:20A – 7:00P Flip collection cages 11A.  weak prelay. Flip cages again 12P. Testing new version GPUv1034 on dense microtubule data (data4). Fixed embryos (15 min fix) 2-4.5 hrs meeting with XZ.  Make slides giving background facts / known research … Continue reading →

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GPU mttubule imaging

Posted on November 23, 2011 by admin
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Tuesday 11/22/11

Posted on November 22, 2011 by admin

10:00 A – 8:00P, 9:00P-10:00P Finishing annotation of revised CheckDotUpDown code to do 3D dotfinding. Running test analysis of MP06 data. New method for finding unique spheres is easier to read but much much slower, need to find some solution … Continue reading →

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Setting up git on new computer to use existing projects

Posted on November 22, 2011 by admin

following this guide: Need Putty.zip from here using latest mysys git on system files. didn’t seem to get the option to use Plink, but I’m used to running push pull from the bash.  It useful to see the gitGUI log from … Continue reading →

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Monday 11-21-11

Posted on November 21, 2011 by admin

10:30A – 10:00P Revising dotfinding and CheckDotUpDown code: rewritten check for duplicate dots in more transparent fashion. updated master in github. Revised new method for improved efficiency to check for duplicate dots.

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Protected: Chromatin dynamics project

Posted on November 19, 2011 by admin

There is no excerpt because this is a protected post.

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Saturday 11/19/11

Posted on November 19, 2011 by admin

9:40 A Finish O/N confocal of MP08.  run successful.  Downloaded data. Confocal analysis of last round of embryo stains.  Need to add post summarizing results. Added Monet to filepath at Github AlistairBoettiger. (laptop communicates as a separate user, AlistairMobile).  Cloned … Continue reading →

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Friday 11/18/11

Posted on November 18, 2011 by admin

10:30A — 11:15 P Overnight confocal quick check data look great. Day 3 in situs (using new chambers). WaP book6, ch23 Labeling secondary antibodies.  all are donkey antibodies from Jackson Immuno.  reported are absorption values from the spec at the … Continue reading →

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Thursday 11/17/11

Posted on November 17, 2011 by admin

10:45 A – 6:30P, 8:30P – 12:00A Overnight confocal imaging failed: did not reactivate 647 and 488 lasers.  Did not reset offset to match starting embryo position?  Substantial drift still observed without def. focus. Staining: shp a-dig, rabbit a-bcd,m a-lamin … Continue reading →

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Wednesday 11/16/11

Posted on November 16, 2011 by admin

10:20 A – 10:00P, 10:20P — 12:20A in situs day 1: sna[D] 2 vials stained with sna-dig, MP09 2 vials stained with y-full-dig, 1 vial 3-7hr wt E(Pc)-dig, tou-bio, en-dnp. vial 2, 3-7hr wt, en-dig, E(Pc)-bio, tou-dnp. don’t remove all … Continue reading →

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