Monthly Archives: August 2012

Protected: sna perturbations update

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Protected: snail promoter data, images from Mounia

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Thursday 08/23/12

9:50 A-7:00P O/N confocal run not completed.  Increased speed to 6, running until 2p. Trying to run daoSTORM3d.  path files all screwed up, won’t work. Fixed path files (hazen has a script which can be called prior to execution). To … Continue reading

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Wednesday 08/22/12

9:25 A – 7:00P, 8:00P – 11:15P breakdown O/N confocal run of m106 (ThsPr minus proximal enhancer) Continue running dot counting on previous mRNA data process new O/N m106 data.  weird image error after image15.  suspect screw-up with new LSM5 … Continue reading

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Harvard FAS research computing

Currently configured on Tuck: copy OpenAuth path files onto Tuck (now in GeneralSTORM) Launch OpenAuth Launch XLaunch use connection 3 (Yari is using 1 & 2).  Shortcut in GeneralSTORM folder. Launch Putty, use saved mysettings connection (default Xlaunch connection 3 … Continue reading

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Tuesday 08/21/12

9:45 A-5:00P, 8:00P-9:45P Write to TRiP about Pc cross to 3x mat Gal4 outcomes (see previous post). Running analysis of Dfd dataset. This dataset seems to run out of memory and freezes matlab.   Should upgrade RAM in Monet. Literature review: … Continue reading

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Protected: H3K27 in vivo structural dynamics

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annotation for 2012-08-16 AbdA embryos

imreg_0 Germband extension emb 1 imreg_1 Germband extension emb 1 imreg_2 hr 20+ emb 1 imreg_3  hr 14+  emb 1 imreg_4  hr 14+  emb 1 imreg_5 Germband retraction emb 1 imreg_12  hr 14+  emb 1 imreg_13 partial emb 4-10 hr.  emb … Continue reading

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Monday 08/20/12

10:00A – 7:00P Flip fly stocks. TRiP lines all tested, don’t plan to keep since no phenotype obvious.  Should keep progeny to test fertility as a possible phenotype. Revising movie2vectorSTORM (see Github updates). Playing with promoter simulations (see posts) Start … Continue reading

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Protected: snail modeling with maternal variation

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