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Monthly Archives: August 2012
Protected: sna perturbations update
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Posted in snail patterning
Tagged images, modeling, sna
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Protected: snail promoter data, images from Mounia
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Posted in snail patterning
Tagged image analysis, images
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Thursday 08/23/12
9:50 A-7:00P O/N confocal run not completed. Increased speed to 6, running until 2p. Trying to run daoSTORM3d. path files all screwed up, won’t work. Fixed path files (hazen has a script which can be called prior to execution). To … Continue reading
Wednesday 08/22/12
9:25 A – 7:00P, 8:00P – 11:15P breakdown O/N confocal run of m106 (ThsPr minus proximal enhancer) Continue running dot counting on previous mRNA data process new O/N m106 data. weird image error after image15. suspect screw-up with new LSM5 … Continue reading
Harvard FAS research computing
Currently configured on Tuck: copy OpenAuth path files onto Tuck (now in GeneralSTORM) Launch OpenAuth Launch XLaunch use connection 3 (Yari is using 1 & 2). Shortcut in GeneralSTORM folder. Launch Putty, use saved mysettings connection (default Xlaunch connection 3 … Continue reading
Tuesday 08/21/12
9:45 A-5:00P, 8:00P-9:45P Write to TRiP about Pc cross to 3x mat Gal4 outcomes (see previous post). Running analysis of Dfd dataset. This dataset seems to run out of memory and freezes matlab. Should upgrade RAM in Monet. Literature review: … Continue reading
Posted in Summaries
Tagged confocal, image analysis, mRNA counting, STORM analysis
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Protected: H3K27 in vivo structural dynamics
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Posted in Chromatin, Microscopy
Tagged images, results, STORM analysis
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annotation for 2012-08-16 AbdA embryos
imreg_0 Germband extension emb 1 imreg_1 Germband extension emb 1 imreg_2 hr 20+ emb 1 imreg_3 hr 14+ emb 1 imreg_4 hr 14+ emb 1 imreg_5 Germband retraction emb 1 imreg_12 hr 14+ emb 1 imreg_13 partial emb 4-10 hr. emb … Continue reading
Monday 08/20/12
10:00A – 7:00P Flip fly stocks. TRiP lines all tested, don’t plan to keep since no phenotype obvious. Should keep progeny to test fertility as a possible phenotype. Revising movie2vectorSTORM (see Github updates). Playing with promoter simulations (see posts) Start … Continue reading
Protected: snail modeling with maternal variation
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Posted in snail patterning
Tagged images, results
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