Monthly Archives: August 2012

Protected: snail modeling update

Posted on August 19, 2012 by admin

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Sunday 08/18/12

Posted on August 18, 2012 by admin

9:40 A-10:20A, 5:00PM-7:00P snail O/N confocal z-offset incorrect.  Should use nuclei to determine top surface, low snail expression levels are an unreliable indicator.  sim 488 channel slightly saturated.  Reconfigured positions 4-13, started run again. 5P reconfiguring snail simulations, see post. … Continue reading →

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Friday 08/17/12

Posted on August 17, 2012 by admin

10:00 A  – 8:45P, 10:00P-11:45P Etching slide 1 from 6/25 in situs, 1 Dfd-dig, tub-bio.  25 min etch. Mostly over-etched.  setting up for STORM imaging of remaining sections:. more snail simulations. Approach 1, see if kicking in a twist activation of … Continue reading →

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Thursday 08/16/12

Posted on August 16, 2012 by admin

10:10 A-4:30P, 9:00P – 11:40 P (8A-10A volleyball practice) etching sample S7 from 6/25 (coverslip 2 from last night).  sample disolved clean away at 55 min.  (previous sample over-etched at 1hr:30, sample before that nicely etched at 1 hr 30, … Continue reading →

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Protected: ANT-C locus K27me3 by STORM try 1

Posted on August 15, 2012 by admin

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Wednesday 08/15/12

Posted on August 15, 2012 by admin

9:45 A – 6:50P, 8:00P – 11:20PM Finish O/N confocal run on slide m105, save data running simulation on full size embryo of snail promoter mutants.  Strong preference for patches.  Earlier activation of repressors supports conditions that create broader snail … Continue reading →

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Protected: tissue specific Methylation state of the BX-C complex by STORM

Posted on August 15, 2012 by admin

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Tuesday 08/14/12

Posted on August 14, 2012 by admin

9:15 A – 7:00 P, 8:30 P – 11:20 P Finish O/N STORM run of Antp/Tub bead images z-calibration first iteration looks horrible.  May need new few guess parameters.  Attempting multiple iterations. simulating snail patterning with different promoters.  Need substantial … Continue reading →

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Monday 08/13/12

Posted on August 13, 2012 by admin

9:00 A – 6:40P, 8:00P – 10:30P Zeiss780 training (just like 710.  use 10% of previous laser power.  Detector will shut off for protection if too much saturation) Sarah Lab meeting wnt signalsomes and purinsomes Adding mol-list to image functionality … Continue reading →

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Saturday 08-11-12

Posted on August 13, 2012 by admin

12:45 P-6:30P, 8:30P-10:30P Analyzing 08-07-12 bead data for STORM4 a match radius of 7 is required for aligning all the beads correctly due to a dramatic rotational offset in the channels.  This results in 20-30 nm 3D alignment. Substantially stronger … Continue reading →

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