Monthly Archives: September 2012

Wednesday 09/19/12

9:30 A – 2:45P Working on GUI somehow the zoom button isn’t getting passed the correct minx and miny, even though these sit in the handles structure and it is getting passed the correct handles.zm !!!! WTF. Rest of navigation … Continue reading

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Tuesday 09/18/2012

9:20 A – 7:30P, call about shoes http://www.scottishclansman.com/brogues.htmlin Vallejo see also for ordering http://www.kilts-n-stuff.com/contact-info.html Banff meeting organization write to co-organizers about feasibility of Feb conf. Pick up flies from mailroom! STORM code development Finalized function GenGaussianSRImage as a wrapper for … Continue reading

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Monday 09/17/12

9:30 AM – 7:30PM, 9:00P – 11:00P Start Unsupervised_Dotfinding for mRNA counting of sog data. Troubleshooting error with list2img for movie2vectorSTORM: not outputting Gaussian image Getting GPU gaussian blobs script to work — test script works great Lab meeting (Shu: … Continue reading

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Sunday 09/16/12

1:00P – 7:00P, 9:00P-10:30P Analyzing data from DaoSTORM run.  InsightM crashed all 750 movies, so unable to compare at the moment.  However the DaoSTORM images look good and qualitatively similar to previous analyses done with insight. Modifying movie2vectorSTORM to deal … Continue reading

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Friday 09/14/12

10:30 AM Still trying to install DaoSTORM on Monet.  See post. Installed CMAKE (go for the exe) Installed LAPACK (finally). Attempting to install mingw-64 bit. After many failed attempts, now seems to be working.  However Lapack still won’t link into … Continue reading

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Installing DaoSTORM for windows

Into the rabbit hole! DaoSTORM requires 64 bit C-libraries, which requires a 64 bit compiler, which to build first requires an installation of a c-32 bit compiler.  Also requires Fortran libraries in Lapack which requires the program CMAKE to install … Continue reading

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oligo PAINT in embryos

DNA FISH / Oligo-PAINT in embryos.  Progress. Note the substantial variation in size.  some of the spots resolve into multiple dense clusters and others are pretty diffuse over a large area. This centromeric AATAT rich repeat covers ~250kb region. Since … Continue reading

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Protected: Tup promoter

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DaoSTORM for Matlab

  mufit starts writing  bin files immediately (good for memory) First we need a watcher: A good introduction to using WMIC http://isc.sans.edu/diary.html?storyid=2376 (Windows Management Instrumentation Command) [status result] = dos(‘wmic process where name=”python.exe” list brief’); running = strfind(result,’python.exe’); Running head-to-head comparison of … Continue reading

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Thursday 09/13/12

9:00 A – 4:00P, + remotely 5:00P-7:20P, 8:20P-11:20PM O/N STORM run still going smooth and in focus at 8:00 AM. O/N STORM run completes on time, 9:30AM.  Break down run turn off scope. Coding Coding matlab wrapper for python / … Continue reading

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