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Monthly Archives: September 2012
Wednesday 09/12/12
9:20A – 7:00P STORM4 status Laser powers: 488 68mW, 561 50mW, 656 78mW. Neither table is floating At first system failed to connect properly to AOTF Fixed AOTF by reboot, but system now fails to connect to focus-lock IR camera. … Continue reading
Coding projects to do:
mRNA counting package change fname to include slidedate folder as well as fileroot in all major scripts: Unsupervised_Dotfinding, Analyze_data, Plot_data scripts STORM analysis Matlab wrapper for multifit Daostorm (to run locally). Finished and running smoothly on Tuck, 09/16/12 Modify insightM … Continue reading
Tuesday 09/11/12
9:10 AM – 7:15 P, 9:00P – 11:55 P Log off Tuck (Reserved for Yari) – 7 Processes all doing final fit on ~500,000 molecule list (about 1 molecule/per sec! These are gonna have to get killed…) Finish O/N STORM … Continue reading
Monday 09/10/12
9:40 A – 5:00P, 8:15P – 11:00P Restart data run on Tuck Transfer files from STORM4 (en STORM from 9/6… oops). Etching slide S6 for imaging today. Move ND filter 5 from STORM2 750 line to STORM4. Colenso also notices … Continue reading
Sunday 09/09/12
4:00P-7:00P, 9:45P-11:30P Organizing snail data in lab notebook summary (see post). Figured out how to add multiple different slideshows to same post (can reference a wordpress object/post by id=xxxx. can also use include = attachment id. (mouse over thumbnail for … Continue reading
Protected: Maps of all snail perturbation data
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Posted in snail patterning
Tagged images, results, sna
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First pass at H3K27me3 imaging at the ANT-C locus in embryos
Posted in Chromatin, General STORM
Tagged H3K27, images, result
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Saturday 09/08/12
10:15 A – 7:00P, 9:00P-10:00P Working with new ways to plot snail data updated annotation on snail modeling and snail Pr/enh data plotting. modeling repressor strength. plotting summary of k27 STORM results at Hox loci (see post) working on plotting … Continue reading
Protected: exploring repression and activation from the same enhancer (with snail)
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Posted in snail patterning
Tagged images, result, sna
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Friday 09/07/12
9:05 A – 8:00 P O/N STORM lost focus attempt to get bead images aborted. Issues with focus lock delayed start and Colenso needs to get on the machine. O/N confocal still had lots of issues with z-focus (apparently not … Continue reading