Monthly Archives: October 2012

Tuesday 10/23/12

Posted on October 23, 2012 by admin

9:20 A – 6:30P, 8:00P-11:00P (remote) S2 cell DNA-FISH labeling Fixing S2 cells for DFISH (1/2x PBT 5% formaldehyde) cells still attached, looks promising. cells preincubated at 92C for 3min, 60C for 20min, moved to RT probes denatured at 92C … Continue reading →

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Monday 10/22/12

Posted on October 22, 2012 by admin

9:20 A – 8:30, 9:30 – 11:10PM Flip collection cage take embryos out of 4C, final hour incubating at RT before washes. snail in situs Draq5 stained and mounted STORM / section in situs / DFISH all in secondaries at … Continue reading →

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Sunday 10/21/12

Posted on October 21, 2012 by admin

11:00 A-3:00P, 7:00P – 9:40 PM upgrade linux laptop to 12.10 Review writing reading /re-reading recent literature White/Barolo/Cohen paper He / Sinha model see post Experiments Rinse out probes from DNA FISH (probes saved – green box) Rinse out probes … Continue reading →

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Saturday 10/12/12

Posted on October 20, 2012 by admin

10:30 A – 7:00P, Experiment planning stuff some more Drosophila embryogenesis staging guides Protocols for culturing S2 cells. DNA FISH centromere labeling test different post-fix conditions and check staining at different post-fix steps to ID loss of DNA-FISH labeling. Incubating … Continue reading →

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Friday 10/19/12

Posted on October 19, 2012 by admin

9:30A – 5:00PM Embryo fixation 9:30A Flip collection plate Making new buffer A fix mix 10 mL, fresh.  (Should have made 100 mL and froze aliquots?) 10:30A Fix embryos.  Now in -20C in MeOH. Write to lab and Francis lab … Continue reading →

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Thursday 10/18/12

Posted on October 18, 2012 by admin

9:40 A – 7:00P, 8:20P – 10:45P Flip collection cage, 9:45A.  Keep plate to make new bottles Pick up more 10x TAE and 20x SSC from stock room Picked up 6 new pints of 100% EtOH. Found issue with K27:H3 … Continue reading →

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Protected: summary results so far on K27me3 dynamics

Posted on October 18, 2012 by admin

There is no excerpt because this is a protected post.

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Protected: Experiments to try later

Posted on October 18, 2012 by admin

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Wednesday 10/17/12

Posted on October 17, 2012 by admin

9:10 A – 6:00P, 9:30P – 11:20 P start thawing DNA Fix Mix at 37C email BIRS team about NIH funding option update Need to coat new coverslips.  Contacting olympus about #1.5 vs #1 coverslips.  (Zeiss recommends only 1.5, strong … Continue reading →

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Aptamers for chromatin

Posted on October 17, 2012 by admin

histone aptamers H4 (+H3) aptamer (publication) 5′- {Alexa Fluor 647} – {Amino Modifier C6 dT} – GGT GGG GTT CCC GGG AGG GCG GCT ACG GGT TCC GTA ATC AGA TTT GTG T – 3′ Need to improve crosslinking of small … Continue reading →

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