Daily Archives: February 23, 2013

Saturday 03/23/13

Posted on February 23, 2013 by admin

10:30 A – 6:20P, 9:30P Embryo collection Flip all fly cages dispose of previous fly plates with new plate recycling and freezing system. Fix embryos 9:30 PM (~3-14 hrs, mostly at raised at 29C). STORM working out parameters for TCEP … Continue reading →

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STORM Buffers

Posted on February 23, 2013 by admin

TCEP Buffer 0.2M Tris pH 9  (200 uL Tris 1M pH 9.0) 1 mM Methyl viologen (10 uL of 0.1 M stock.  Currently make fresh from powder. Toxic) (25.7 mg / mL) 1 mM Ascorbic Acid (10 uL of 0.1 … Continue reading →

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