Daily Archives: February 28, 2013

Thursday 02/28/13

Posted on February 28, 2013 by admin

10:20 A -6:45P, 8:15P – 11:55P (workout 8:30-10:20A) Probe Making (1P-6P @ Longwood) emPCR for 100kb yellow, green and black regions (at Wu lab) see new protocol 35-50 ng/uL yield (11 uL total volume) Notebook Looking in to version managing … Continue reading →

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Large Scale PCR for probe synthesis

Posted on February 28, 2013 by admin

Part 1 – PCR amplification Notes: We recommend setting up 10 ml of PCR master mix and aliquoting it into a 96-well plate, 100 µl per well. This protocol is scaled for a starting amount of 10 ml; simply scale … Continue reading →

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Emulsion PCR

Posted on February 28, 2013 by admin

For initial amplification from Oligo-Library. Setup Components Glass vials kept at -20C. magnetic stir plates at 4C/in cold room (capable of 1000 rpm) emulsion oil mix (can make in 50mL volume and store at 4C): 80 uL ABIL EM90 (request … Continue reading →

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