Monthly Archives: April 2013

Protected: project 2 coding update

Posted on April 19, 2013 by admin

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Thursday 04/18/13

Posted on April 18, 2013 by admin

9:30 A – 9:00 P, 9:30 P – 1:00 A Computantional OligoPaint probe design Research Computing (RC) doesn’t intend access nodes to be used to submit batch jobs bsub batch submission not working. Wrote to RC for help. Working on … Continue reading →

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Wednesday 04/17/13

Posted on April 17, 2013 by admin

9:40 A – 7:15 P, 8:00 P – 8:30 P, remotely, 9:00 P – 10:30 P Coding see additional project notes for today. Got OligoArray working on Cajal, figuring out ideal batch size and memory limits: Processes eat ~1.8 GB … Continue reading →

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Protected: project 2 notes

Posted on April 17, 2013 by admin

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qubit measurements

Posted on April 17, 2013 by admin

All numbers in ng/uL (equivelantly ug/mL). Original volume is 1 mL so this equivelantly DNA mass. Though some samples were split multiple ways, EtOH is 2:1 with vs without glycogen. Filtration was done with 150 uL of 1 mL PCR. … Continue reading →

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Protected: project 2 coding

Posted on April 16, 2013 by admin

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Tuesday 04/16/13

Posted on April 16, 2013 by admin

9:30 A – 8:00 P, 9:00P – 12:00 A OligoPaints Set up EtOH precipitation of samples 1-5. In -20C, ~10:00 A. Pour 5% agarose Na-Borax gel to try to diagnose issue with ladder: not all bands showing up on urea … Continue reading →

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Monday 04/15/13

Posted on April 15, 2013 by admin

9:30 A – 8:00 P, 9:00 P – 11:55 P Meetings group meeting (notes) journal club (notes) discussion with Xiaowei (notes) Gels At least 3 ways to screw up gels APS really needs to fresh, (try 140 uL instead of … Continue reading →

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Protected: new project 2

Posted on April 15, 2013 by admin

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Structural and molecular interrogation of intact biological systems

Posted on April 15, 2013 by admin

CLARITY hydrogel electrophoretic tissue transmutation. Nature Paper cross link gel. Add ionic detergent (SDS micelle), extract all membranes in electrophoresis tissue becomes much clearer, can image deep proteins still there. do they demonstrate nucleic acids? – doesn’t look like it. … Continue reading →

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