Monthly Archives: April 2013

Thursday 04/04/13

Posted on April 4, 2013 by admin

9:30 A STORM Finish O/N STORM run of 3x Cy5 512×512 on STORM2 # Figure out were to transfer data Modeling # Reply to Chris STORM Analysis Implement automatically detected, variable size window to STORMrender. A lot of Ph movies … Continue reading →

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Wednesday 04/03/13

Posted on April 3, 2013 by admin

9:45 A – 8:00 P, 9:30 P – 12:00 A General Send notes on sog data to Mounia Training on Qbit. Important point: measure samples between 2 and 2.5 minutes after mixing in reaction dye. STORM Analysis Manual analysis of … Continue reading →

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Tuesday 04/02/13

Posted on April 2, 2013 by admin

9:45A – 5:00P, 8:00P – 10:00P Modeling Exploring parameter space of site-interaction-number models. Bursty regime: fraction of time in the transcribing state is low (kon/(kon+koff)) Transcription rate in the on phase cooperativity noise Most obvious at low induction In general … Continue reading →

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Monday 04/01/13

Posted on April 1, 2013 by admin

10:00 A – 7:00P, 9:00P-10:00P General hp psc1200 printer not printing color correctly. lab meeting (notes) journal club (notes) Review writing Insight 1: enhancer switching between obstructed and unobstructed case is the bursting model with different tx rates from ‘off’ … Continue reading →

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oligopaint probe making planning

Posted on April 1, 2013 by admin

emPCR ready libraries: AbdA AbdB Ubx en-locus 100kb Black 100kb Yellow 100kb Green 100kb Synthesis schemes: Teritaries + Nicking PCR using extended primers = Forward primer (21bp) + Secondary binding site (32 bp) Nick off R primer Denaturing PAGE Gel … Continue reading →

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journal club: single sperm sequencing

Posted on April 1, 2013 by admin

Probing Meiotic recombination and aneuploidy single sperm cells (Xie lab) haplotyping homologous recombination, major source of genetic diversity determine haplogenotype of each cell. Sequence diploid, not sure which SNPs are on which homologous copy (i.e. which are linked) HAP map … Continue reading →

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Protected: lab meeting 04/01/13

Posted on April 1, 2013 by admin

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