Daily Archives: August 30, 2013

RT reactions

Posted on August 30, 2013 by admin

background prep for RT reactions resuspended common-P1 at 1000 uM concentration 405 primer from Invitrogen not arrived yet compare superscriptII to superscriptIII in yield superscriptII protocol superscriptIII protocol 85 bp 1000 ng/uL ~ 35 pmol per uL = 350 pmol … Continue reading →

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Library analysis

Posted on August 30, 2013 by admin

Labeled version of gel: thoughts small sub-libraries preferentially lost? is this because we only get a fraction of the probes in each sublibrary to amplify? more likely a relative abundance issue — should have substantially more mass in large libraries. … Continue reading →

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Friday 08/30/13

Posted on August 30, 2013 by admin

8:30a-5:00p Goals for today RNA clean up of pilot Lib2 templates. comparison of SuperscriptII and SuperscriptIII RT yields plate scale in vitro transcription? Align multi-day stain get comments on review Coding writing script to aid finding cell matches from Mosaics … Continue reading →

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