Daily Archives: August 28, 2013

Wednesday 08/28/13

Posted on August 28, 2013 by admin

8:00a – 7:15p, 10:15p-11:00p Probe making set up in vitro Tx reactions run new gel with ladder: G1 and G7 look blank, others look good (a bit troubling, since G7 nanodrops at ~75ng/uL even though G1 goes blank). Full library … Continue reading →

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plate PCR for 82 libraries

Posted on August 28, 2013 by admin

for each sublib (50 uL reaction) 19 ddH2O 5 uL 5 uM common 1 uL of 1:10 diluted library 25 uL Phusion master 0.25 uL of 200 uM T7 lib specific primer master mix (83x) 1577 uL ddH2O 415 uL … Continue reading →

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T7 in vitro Tx

Posted on August 28, 2013 by admin

Library2, samples G1-8 skip sample G1, no DNA recovery NEB For each sub-library, mix the following in a single PCR tube or well of a PCR strip or plate 8 uL DNA template + 2uL ddH2o 1.5 uL 10X T7 … Continue reading →

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PCR

Posted on August 28, 2013 by admin

Amplifying G1-8 using T7 primers for each sublib 18.5 ddH2O 5 uL 5uM common 1 uL library (cut to .5, at 1uL I can only do each lib once) 25 uL Phusion master master mix (9x) 166.5 uL ddH2O 45 … Continue reading →

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