Monthly Archives: September 2013

Friday 09/20/13

Posted on September 22, 2013 by admin

10:15a – 6:30p, 8:30p-10:00p Planning Discussion meeting with Ting 10:15a-11:15a (see notes) Planning embryo Pc next steps (see notes) Writing continue shortening BJ review Re-read and submit referee comments. Added extensive additional discussion on conclusions. Experiments with Bogdan Set up … Continue reading →

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Thursday 09/19/13

Posted on September 19, 2013 by admin

9:00a – 7:45p, 10:00p – 11:00p Writing Referee work working remotely (9a-12p) write up comments BJ review Goal: Trim down BJ review by 3 of 16 pages started trimming from beginning. 1 full page shorter. mRNA indexing human genome 22917 … Continue reading →

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Wednesday 09/18/13

Posted on September 18, 2013 by admin

9:15a – 11:30p Writing working on BIRS workshop report (9:15a- 12p) Probe making / training with Bogdan test PCR from yesterday common primer concentration was a bit low gel didn’t fully dissolve, might have affected gel running: Repeat PCRs. Repeat … Continue reading →

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Tuesday 09/17/13

Posted on September 17, 2013 by admin

Library negative controls Get 10% bleach for spray bottle. Bleach all pipettes. Get all new tips 25 uL Phusion reactions 10 uM primer dilutions Fresh library 1:100 dilution 1 uL + 1.25 uL 10uM common Fresh library 1:100 dilution 1 … Continue reading →

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Sunday 09/15/13

Posted on September 15, 2013 by admin

Shvartsman Bomyi lim (grad student) Intro Michaelis Menten / invertase. disrupted last bastion of vitalism did not know what enzyme was, could not obseve ES complex, proof was purely kinetic enzymes modyifing proteins may have less specificity — region bound … Continue reading →

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Saturday 09/14/13

Posted on September 14, 2013 by admin

Zenklusen Intro use nascent mRNA to measure initiation frequency live imaging short memory of how many transcripts initiated MS2 RNA labeling — look at intensity fluctuations initiations are uncorrelated. variable elongation rates coordinated with cell cycle (faster in G2/M) MS2 … Continue reading →

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Thursday 09/12/13

Posted on September 12, 2013 by admin

STORM analysis set up splitdax analysis for last of unsplit G4 data (somehow a lot of 0 bit movies got written). set up DaoSTORM analysis for G4 data Travel to Banff for BIRS conference Writing reading latest paper for review … Continue reading →

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Wednesday 09/11/13

Posted on September 11, 2013 by admin

9:30a – 11:30p Review submit review submit copyright agreement BIRS draft email of final reminders contact Vistas Dining hall about meal costs and reserving tables Print driving directions to and from Airport/Banff, Lakes, and hotel. Print hotel receipt Probe making … Continue reading →

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Tuesday 09/10/13

Posted on September 10, 2013 by admin

8:50a – 4:40p STORM Finish O/N STORM of G4 start splitdax Probe making RNA cleanup using Beckman RNAClean XP beads. Samples F1-F5, F7-F9. Tube format F1 yield ~1,500 ng/uL in 25 uL (37.5 ug of RNA). F2 yield ~700 ng/uL … Continue reading →

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Monday 09/09/13

Posted on September 9, 2013 by admin

9:00a – 7:30p, 8:00p-11:30p Goals Re-read and submit review! slides for meeting with XZ. Meetings lab meeting journal club project 2 meeting (see posts) File management delete raw dax for movies separated by splitdax copy files Review MiKTeX updater broke … Continue reading →

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