Monthly Archives: October 2013

Protected: Ph Meeting Notes

Posted on October 11, 2013 by admin

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Thursday 10/10/13

Posted on October 10, 2013 by admin

10:00a – 8:30p, 10:00p – 12:00a Goals finish abstract for Alvaro work on slides for Stowers STORM with Bogdan finish F1, start coverslip 4 STORM data processing with Bogdan Oligo Prep G3-G5 P1A405, P2Cy3, P4Cy3 samples Hybridizations for next small … Continue reading →

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Abstract for Seminar at Rowland Inst.

Posted on October 10, 2013 by admin

Title: Quantification and Analysis of Intrinsic Variation in Gene Expression during Embryonic Development Alistair Boettiger Harvard University Abstract: The molecular scale interactions responsible for turning genes on and off have an inescapable degree of randomness — a consequence of their … Continue reading →

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Wednesday 10/09/13

Posted on October 10, 2013 by admin

9:00a – 12:35a Goals work on Presentation for Stowers STORM Ran out of disk space, crashed O/N Run lost focus anyway immediately after position 0. Probably not enough oil. Possibly positions were chosen too far apart. Need to explain these … Continue reading →

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Revised Fast OligoPaint Synthesis

Posted on October 9, 2013 by admin

Jeffrey Moffitt, Alistair Boettiger, Xiaowei Zhuang jeffmoffitt@gmail.com alistairboettiger@gmail.com Version 2.0: October 2013 Purpose and Overview This protocol describes the construction of OligoPaints FISH probes from a complex oligo library via reverse transcription of an RNA intermediate. By exploiting the high … Continue reading →

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Tuesday 10/08/13

Posted on October 8, 2013 by admin

9:40a – 10:00p Goals Stop O/N STORM run send code to Jeff (11a, dentist) Talk title for Alvaro STORM Stop O/N STORM run IVT reactions out of High Yield T7 buffer Still have 2 vials of T7 High Yield Pol … Continue reading →

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Monday 10/07/13

Posted on October 7, 2013 by admin

9:45a – 8:00p, 1:00a – 2:00a General Group meeting cancelled XZ meeting cancelled Ph modeling Discuss analysis methods with Jeff Moment comparisons. nmin / cv^2 way different than 1, definetely not exponentional (also obvious by visual inspection, median 4-7, mean … Continue reading →

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Saturday 10/05/13

Posted on October 5, 2013 by admin

(WFA, 8:30a-6:30p) STORM Finish O/N STORM of G05 Set up new STORM of E06-E(Pc) backgroundy? faint spots per cell, too many? Maybe E2 also not working? Some cells have clearer dots? Lets wait to see the topology and do a … Continue reading →

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Friday 10/04/13

Posted on October 4, 2013 by admin

9:30a-4:30p, 7:00p-12:00a Goals Figures for Ajaz! Work on Banff report Start presentation for Stowers order Illumina MiSeq components Order NEB primers PCR Overnight power failures? Tuck auto-restarted in night. Overnight analysis aborted/lost Monet auto-restarted in night. Overnight analysis aborted/lost IR … Continue reading →

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Thursday 10/03/13

Posted on October 3, 2013 by admin

10:00a – 7:00p, 10:00p – 11:50p Goals today prep slides for meeting with XZ 10 cycle PCR with 2 uL of product to add on NEB adapters STORM of more black regions reply to Nicole and Ajaz about Ph Literature … Continue reading →

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