Monthly Archives: December 2013

Wednesday 12/11/13

Posted on December 11, 2013 by admin

11:15a – 11:45p Ph Project Highly variable radius of gyration measurements for case with binding — suggests not equilbirated. Binding strength probably much to high. Up to .90, run again. Also increased density of K27me3 sites from 50% to 80%, … Continue reading →

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Tuesday 12/10/13

Posted on December 10, 2013 by admin

(finally recovering from flu) 11:00a – 9:00p Probe making run denaturing gel G5 looks weird – what’s with the double band?! maybe give this a test anyway. primers are different lengths, but that doesn’t explain why both primer sets have … Continue reading →

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Protected: project 2 update 12/10/13

Posted on December 10, 2013 by admin

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Friday 12/06/13

Posted on December 6, 2013 by admin

9:50a – 10:45p Goals Presentation prep for meeting with XZ (meeting rescheduled) Coding: work on new z-calibration algorithm add run silent options to STORMrender update template ini and xml parameters to contain new ROI defaults Probe Making HPLC cy7 secondary … Continue reading →

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HPLC 12/06/13

Posted on December 6, 2013 by admin

DNA labeling 60 nmol of DNA in 100 uL .1M NaHCO3 (100 mg in 12 mL) .1 – .2 mg dye in 50 uL DMSO (50 uL of 1-2 mg / mL) React at 37C for 1-2 hrs (shake occassionally). … Continue reading →

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Protected: Jeff Practice Talk

Posted on December 6, 2013 by admin

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Thursday 12/05/13

Posted on December 5, 2013 by admin

9:30a – 12:20a Goals Restart analysis for project 2 Probe making: check PCRs, continue if good, restart if not. launch STORM analysis for last few days of imaging project 2 see notes. Chromatin Project Probe Making Test PCRs Clean, reorganize, … Continue reading →

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Protected: project 2 update

Posted on December 5, 2013 by admin

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Wednesday 12/04/13

Posted on December 4, 2013 by admin

9:40a – 11:30p Goals show Hao new project 2 results RNase-Away / bleach treat everything on my bench fix and stain new RNA cells. Allow 1 hour prehybe. Project 2 See notes Chromatin colors, double stain prep Probe organization reorganized … Continue reading →

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Tuesday 12/03/13

Posted on December 3, 2013 by admin

9:15a – 11:15p Goals Call Dad, happy birthday Analyze Ph polymer simulations for meeting with Ting. STORM Finish D07 on STORM2 (9 kb green, transcribed, RNaseA treated). Looks transcribed – variable spots, larger than expected for only 9 kb maybe? … Continue reading →

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