Monthly Archives: April 2014

Saturday 04/12/14

9:30a – 4:00p Computer Management attempting to give users rights to share their hard-drives on the network without having full Admin capabilities to do anything they want. Tried adding my ‘User’ account to these groups “Performance Monitor Users” — can … Continue reading

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Friday 04/11/14

9:30a – 10:00p Goals Need to finish review Need to make slides for symposium at Penn send revised Troubleshooting sudden failure in staining Troubleshooting staining Background of problem: Stained new cells on Saturday, went to image these on Monday — … Continue reading

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Additional Windows Server 2012 notes

On Windows Server 2012, domain users are not allowed to log on locally by default. To edit this, follow these directions. Add Domain Users. Can also edit other permission logs per user here.

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Thursday 04/10/14

10:15a – 11:00p Presentation Prep XZ wants to meet at 3P, need to make slides (for project 2) see more project 2 protected notes. Troubleshooting stains sample E03 (previously stained beautifully) failed to work on new cells. Not a simple … Continue reading

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Wednesday 04/09/14

9:30a – 8:30p Chromatin labeling stains failed again. No stains yet detected with this batch of cells. maybe it’s a cell prep issue? This F07 probe worked fine before. maybe some reagents went bad in the cell prep? Make new … Continue reading

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Tuesday 04/08/14

9:30a – 11:00p Goals reschedule Dentist try 2, Cajal windows upgrade Need to finish review / referee paper Set up STORM imaging of E3 Test new hybes Finish writeup #2 draft for XZ. sent back to team for comments Start … Continue reading

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Monday 04/07/14

9:30a – 11:30p Meetings lab meeting (see protected notes) my journal club presentation (see google-docs presentation) initial planning meeting with Steven — need joint write-up, goal to write by Wed Writing finalizing write-up 1 for XZ with Jeff. working on … Continue reading

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Protected: project 2 notes for XZ

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Protected: Lab Meeting 04/07/14

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hybes, 04/05/14

Chromatin staining, investigating black-yellow interactions Setup Repeating dual-color hybes with new aliquot of S3 750 labeled secondary. Hopefully this fixes the low specific signal 750 problem. found new hybe dilution buffer not well mixed — dextran sulfate sitting at bottom. … Continue reading

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