Monthly Archives: June 2015

Friday 06/19/15

Posted on June 19, 2015 by admin

9:30 am – 5:00 pm Meetings lab meeting (see notes) journal club (see notes) RNAi prep dsRNA template gel (should poor new gel, lanes running funny in old reheated gel. sample order: 1-8 = (Kc-DNA): Su(z)12, SCE, Ph-p, Ph-D, (YW-DNA): … Continue reading →

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Journal Club: Expansion Microscopy 06/19/15

Posted on June 19, 2015 by admin

Chen, Tillberg and Boyden, Expansion Microscopy presented by Yari approach label sample gelation in polyectrolye network proteolysis tissue / polymer sample expand in water image super absorbant polymers (used in diapers) sodium acrolyate + acrylomide special probes required. Can’t just … Continue reading →

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Protected: Group meeting, 06/19/15

Posted on June 19, 2015 by admin

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Thursday, 06/18/15

Posted on June 18, 2015 by admin

9:30 am – 10:45 pm To do submit progress report to DR. (done) email Dell about RAM (done. Dell is very slow with reply. Probably should just go Amazon) email DA about letter (with CV) (done) work on slides (postponed) … Continue reading →

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Wednesday 06/17/15

Posted on June 17, 2015 by admin

9:30 am – 6:20 pm MERFISH finalizing presentation of comparison of pipeline5 and pipeline4 L8 comparison failed to save before I excited to allow JM sufficient RAM to run. Should have checked first! RNAi primers shipped today, should be here. … Continue reading →

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Tuesday 06/16/15

Posted on June 16, 2015 by admin

10:30 am – 7:00 pm Meetings University IP lawyer (12pm) discuss oligopaints with JG Writing started revising DR progress report let’s postpone this till after IP discussions start work on DF application (not begun) Presentations start making slides for ASBMB … Continue reading →

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Monday 06/15/15

Posted on June 15, 2015 by admin

10:00 am – 5:00 pm Referee (10:00 am – 1:30 pm) finished reading article finished writing comments summarized comments in review, summarized article contributions submitted to editorial office. Prep for RNAi experiments Primers look up primers on genomernai.org/v14/ order primers … Continue reading →

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Sunday 06/14/15

Posted on June 14, 2015 by admin

12:00 pm – 12:45pm, 2:30 pm – 7:00 pm Animal culture flip fly plate, 12:30 pm passage cells to 75 mm^2 culture flask. Density looks good. flyMERFISH designing fly transcriptome using Jeff’s new pipeline (as an exploration of new pipeline … Continue reading →

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Saturday 06/13/15

Posted on June 13, 2015 by admin

12:00am -7:00am, 11:30am-7:00pm, Manuscript finished submission, ~7:00 am. yay! New material to work with: Embryos flipped fly plate, 12:30 pm. plan: 3 hr collection + 2 hr age -> 5:30 pm flipped cage again 3:30 pm depolymerizing formaldhyde at 70C … Continue reading →

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PcG perturbation experiment planning

Posted on June 13, 2015 by admin

RNAi Cavalli lab (Gonzalez et al) do RNAi based visual screen of RNAi knockdown, look at Pc. distributions of Pc fluorescence across cells in Pc knockdown is pretty tight — the remaining variability could be background auto-fluorescence. This looks promising. … Continue reading →

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