Tag Archives: STORM

Thursday 11/01/12

Posted on November 1, 2012 by admin

9:30 A – 7:30P, 8:30P- 12:00A Software install on STORM2.  Hal can’t detect drivers for national instruments panel. Overview of software install: see post Fly work Collect virgins Sort and cross Espl/sim flies Clear MTDs into 2 new bottles, move … Continue reading →

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oligo PAINT in embryos

Posted on September 13, 2012 by admin

DNA FISH / Oligo-PAINT in embryos.  Progress. Note the substantial variation in size.  some of the spots resolve into multiple dense clusters and others are pretty diffuse over a large area. This centromeric AATAT rich repeat covers ~250kb region. Since … Continue reading →

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Wednesday 09/12/12

Posted on September 12, 2012 by admin

9:20A – 7:00P STORM4 status Laser powers: 488 68mW, 561 50mW, 656 78mW. Neither table is floating At first system failed to connect properly to AOTF Fixed AOTF by reboot, but system now fails to connect to focus-lock IR camera. … Continue reading →

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Monday 09/10/12

Posted on September 10, 2012 by admin

9:40 A – 5:00P, 8:15P – 11:00P Restart data run on Tuck Transfer files from STORM4 (en STORM from 9/6… oops). Etching slide S6 for imaging today. Move ND filter 5 from STORM2 750 line to STORM4. Colenso also notices … Continue reading →

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Thursday 09/06/12

Posted on September 6, 2012 by admin

9:50 A – 10:00 P InsightM overloads Tuck from O/N data analysis. Canceled all further submissions to que to allow memory / CPU to free up for other users during the day. InsightM also crashing itself due to memory overload … Continue reading →

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Monday 09/03/12

Posted on September 3, 2012 by admin

10:00A – 6:30P, 7:50P – 11:30P Order flies from Bloomington, sim deletion: 7646, Espl rv1: 199 # start expanding sna[18] and double balancer on 3rd. request Esc mutant flies from Vivek wrote to Markstein, try to get in touch with TRiP … Continue reading →

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Friday 08/31/12

Posted on August 31, 2012 by admin

9:30 A – 7:30P, 10:30 – 12:50A Break down O/N mp10a run. save mp07 data.  All look good, 7 images finished completely, last image starts at the top of the mRNA field. etching S1 AbdA, AbdB, Ubx, K27me3. Issues booting … Continue reading →

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Wednesday 08/28/12

Posted on August 29, 2012 by admin

9:50 A-7:00P, 8:30P – 11:50P pick up drive from HCBI STORM4 lost focus O/N.  Reset focus, continue imaging. Trying to get good matches for pnrPr dynamics in simulation, not just end states. Dyes not switching well in 15 min etched … Continue reading →

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Tuesday 08/28/12

Posted on August 28, 2012 by admin

9:30 A – 7:00PM, 7:45P – 12:00A 20 min etch Pc-647 sample.  Turnkey inspection looks over-etched, most embryos quite sparse. Forgot that previous MP07 samples have Draq5 not DAPI.   Extracting nuclear masks manually through inverting DAPI channel and using … Continue reading →

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Sunday 08/26/12

Posted on August 26, 2012 by admin

3:30 PM -:715P, 8:30P – 10:30P STORM run lost focus.  Haven’t analyzed yet at which position / how many movies lost. Setup new collection of positions.  All pretty old embryos in this batch, K27me3-647 stain doesn’t look great, channel has … Continue reading →

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