Tag Archives: coding

Thursday 08/23/12

Posted on August 23, 2012 by admin

9:50 A-7:00P O/N confocal run not completed.  Increased speed to 6, running until 2p. Trying to run daoSTORM3d.  path files all screwed up, won’t work. Fixed path files (hazen has a script which can be called prior to execution). To … Continue reading →

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Wednesday 08/22/12

Posted on August 22, 2012 by admin

9:25 A – 7:00P, 8:00P – 11:15P breakdown O/N confocal run of m106 (ThsPr minus proximal enhancer) Continue running dot counting on previous mRNA data process new O/N m106 data.  weird image error after image15.  suspect screw-up with new LSM5 … Continue reading →

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Harvard FAS research computing

Posted on August 22, 2012 by admin

Currently configured on Tuck: copy OpenAuth path files onto Tuck (now in GeneralSTORM) Launch OpenAuth Launch XLaunch use connection 3 (Yari is using 1 & 2).  Shortcut in GeneralSTORM folder. Launch Putty, use saved mysettings connection (default Xlaunch connection 3 … Continue reading →

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Monday 08/20/12

Posted on August 20, 2012 by admin

10:00A – 7:00P Flip fly stocks. TRiP lines all tested, don’t plan to keep since no phenotype obvious.  Should keep progeny to test fertility as a possible phenotype. Revising movie2vectorSTORM (see Github updates). Playing with promoter simulations (see posts) Start … Continue reading →

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Thursday 08/16/12

Posted on August 16, 2012 by admin

10:10 A-4:30P, 9:00P – 11:40 P (8A-10A volleyball practice) etching sample S7 from 6/25 (coverslip 2 from last night).  sample disolved clean away at 55 min.  (previous sample over-etched at 1hr:30, sample before that nicely etched at 1 hr 30, … Continue reading →

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Monday 08/06/12

Posted on August 6, 2012 by admin

9:45 A – 5:00P, 10:00P – 12:25A Finish O/N confocal run of slide 3 (Tub + Antp) Copying data from Alistair6 to Alistair5 Lab meeting (Wenqin) Troubleshooting issue with Steve output – not perfect linear relation between microns and pixel … Continue reading →

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Saturday 08/04/12

Posted on August 4, 2012 by admin

10:00A-2:45P, 5:20P-7:00P, 8:30P-10:30P in situs day 3 (triple stain try 2). STORM imaging slide 2 from 6/24 stains (Antp) mount samples 1-5 + centromere/K27/Dm01 v2, post-fix rest. Hal running smoothly. Imaging conventional images of each section just prior to STORM … Continue reading →

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Tuesday 07/24/12

Posted on July 24, 2012 by admin

10:00A – 12:30A Revising snail kinetics paper for NSMB Revised Supp Fig 4, revised kinetics paragraph for multicopy.  Recomputed approach to saturation regime controlled by intrinsic kinetics of Pol II. confocal imaging of sample 1 from Mounia KE repeating PCR for new … Continue reading →

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Wednesday 07/18/12

Posted on July 18, 2012 by admin

10:10A – 9:30P working on new SDB talk (kinetics, no bistability) Issue with text animation.  Just on windows system. working on bead fitting.  06-25-beads (100nm) fit pretty well, see all 5 regions in most channels (vis have 4 clear and … Continue reading →

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Monday 07/09/12

Posted on July 9, 2012 by admin

9:50 A – 6:50 P remove slide 3 of H3K4-section from 60C heat block, add to slides. Hazen Lab meeting 3D viewing in Insight:  ctrl+shift+x, select ‘no’, ctrl+x gives 3D viewer.  Need to have color shading as percentage toggled on … Continue reading →

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