Tag Archives: coding

Friday 03/20/12

Posted on April 20, 2012 by admin

11:55 A – 8:00P, 9:30 – 11:30P (volleyball 8:50A-11:50A) need missing reference letter and electronic submission for ASC app! running STORM analysis on 04/05/12 En-tou-Epc-H3 data. Rerunning chromatic alignment on 04/14/12 en-Epc conventional data working on slides for group-meeting presentation

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Wednesday 04/18/12

Posted on April 18, 2012 by admin

9:45 A -8:00P, 9:30P-11:00P Running alignment on all E(Pc)-en-H3 data Used H3 4-color experiment beads.  should use correct slide beads. Running bead analysis for all E(Pc)-en-H3 data. Create new fxns for bead runs.

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Tuesday 04/17/12

Posted on April 17, 2012 by admin

9:40 A – 8:00P, 9:40P-11:45P Finish O/N storm run Running H3 4-color STORM data processing on Cajal.  Z-calibration analysis with autoZ script gives crazy results.  Using previous parameter files for analysis. Zcal error created from system grabing data files instead … Continue reading →

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Monday 04/16/12

Posted on April 16, 2012 by admin

9:50 A – 12:20 A Lab meeting Yari Lab discussion on STORM scopes: should be used all night, should upgrade STORM3 so more users can do their experiments — need 488 line and 647 line. Wenqin’s Thesis defense Journal club … Continue reading →

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Thursday 04/12/12

Posted on April 12, 2012 by admin

9:20 A – 11:50 P Both large and small plates look good. large plates a bit sparse but still good colonies to chose from. Benny Shilo seminar coding automated iterations of z-fitting in matlab. oligo-paint- good staining!  high DNA background … Continue reading →

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Friday 03/30/12

Posted on March 30, 2012 by admin

9:20A — 8:00 P Shuttle to Med campus to see Xiaochen talk (White lab student interviewing for Perrimon / Bulyk labs) Very clean CTCF data.  Should try this antibody in STORM Finalize ACS application.  Submitted paper copy and FEDEX’d. Still … Continue reading →

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Tuesday 03/27/12

Posted on March 27, 2012 by admin

11:00A – 8:00P, Coding Accelerating integrated 4 color chromatic, drift correction into code to convert molecule list to corrected tif images. Notes from STORM presentation STORM2 power report, At 40 x 40 um at 60 Hz: 20 mW 750 at … Continue reading →

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Monday 03/26/12

Posted on March 26, 2012 by admin

10:00 A – 7:30P, 9;30P-11:00P Lab Cleaning List  of tasks [as reference for next time] Gel Bench (Upstairs) pH Bench (Upstairs and Downstairs) miniprep Bench (Upstairs) weigh station (Upstairs and Downstairs) Clean Fume hood (Upstairs and Downstairs) Clean cell-culture hood (Upstairs and … Continue reading →

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Wednesday 03/21/12

Posted on March 21, 2012 by admin

12:00P – 9:30P (9A-12P lab volleyball practice) in situs day 2 protein stains wash out primaries, post-fix, add secondaries. started new page, see record: Kill old bottles of MP09, yw.  Need to set up new bottles STORM imaging of 3Mb … Continue reading →

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Tuesday 03/20/12

Posted on March 20, 2012 by admin

10:00 A – 8:00P, 9:30P-10:30P In situs day 1 Flip fly cages, 10:30A Attempted sodium borohydride treatment of half of samples.  Left sealed on shaker — vials exploded, rocket off of shacker from underneath tin foil cover half way across … Continue reading →

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